利用汉逊德巴利酵母处理味精废水生产微生物油脂。
Microbial treatment of the monosodium glutamate wastewater by Lipomyces starkeyi to produce microbial lipid.
机构信息
Shandong Key Laboratory of Water Pollution Control and Resource Reuse, School of Environmental Science and Engineering, Shandong University, No. 27 Shanda South Road, Jinan 250100, PR China.
出版信息
Bioresour Technol. 2012 Feb;106:69-73. doi: 10.1016/j.biortech.2011.12.022. Epub 2011 Dec 13.
The monosodium glutamate (MSG) wastewater as a medium was treated by Lipomyces starkeyi to produce microbial lipid in the study. The effect of related factors (initial glucose concentration, inoculation concentration, initial culture pH, and cultivation time) on biomass, lipid production and lipid content was discussed, respectively. According to the experiments, the optimal fermentation conditions were determined: addition of 80g/L glucose, 10% inoculation concentration, initial pH about 5.0, incubation time 96h. Under this condition, the biomass production reached up to 4.61g/L, lipid production and lipid content was 1.14g/L and 24.73%, respectively. Simultaneously, protein and COD removal rate was 78.60% and 74.96%, respectively. The main composition of fatty acid in the resultant lipid was analyzed by gas chromatography-mass spectrometry, which showed: oleic acid (C18:1) 35.85%, palmitic acid (C16:0) 19.91%, palmitoleic acid (C16:1) 17.65%, and myristic acid (C14:0) 16.03%.
在本研究中，采用谷氨酸单钠（MSG）废水作为培养基，通过斯达油脂酵母生产微生物油脂。分别探讨了相关因素（初始葡萄糖浓度、接种浓度、初始培养 pH 值和培养时间）对生物量、油脂产量和油脂含量的影响。根据实验结果，确定了最佳发酵条件：添加 80g/L 葡萄糖、10%的接种浓度、初始 pH 值约为 5.0、培养时间 96h。在此条件下，生物量达到 4.61g/L，油脂产量和油脂含量分别为 1.14g/L 和 24.73%。同时，蛋白质和 COD 的去除率分别达到 78.60%和 74.96%。通过气相色谱-质谱联用仪对所得油脂的脂肪酸组成进行了分析，结果表明：油酸（C18:1）占 35.85%，棕榈酸（C16:0）占 19.91%，棕榈油酸（C16:1）占 17.65%，肉豆蔻酸（C14:0）占 16.03%。
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