Department of Bioprocess Engineering and Biotechnology, Federal University of Tocantins (UFT), Badejos Street 69-72, Jardim Cervilha, Gurupi, TO, 77404-970, Brazil.
Department of Process Engineering (DEPro), School of Chemical Engineering, State University of Campinas (UNICAMP), Albert Einstein Avenue, 500, Zeferino Vaz University City, Campinas, SP, 13083-852, Brazil.
Biotechnol Lett. 2021 May;43(5):967-979. doi: 10.1007/s10529-021-03080-7. Epub 2021 Jan 31.
The extraction of the hemicellulose fraction of sugarcane bagasse (SCB) by acid hydrolysis was evaluated in an autoclave and a Parr reactor aiming the application of the hydrolysate as a carbon source for lipid production by Lipomyces starkeyi.
The hydrolysis that resulted in the highest sugar concentration was obtained by treatment in the Parr reactor (HH) at 1.5% (m/v) HSO and 120 °C for 20 min, reaching a hemicellulose conversion of approximately 82%. The adaptation of the yeast to the hydrolysate provided good fermentability and no lag phase. The fermentation of hemicellulose-derived sugars (HH) by L. starkeyi resulted in a 27.8% (w/w) lipid content and Y of 0.16 g/l.h. Increasing the inoculum size increased the lipid content by approximately 61%, reaching 44.8% (w/w).
The hemicellulose hydrolysate from SCB is a potential substrate for L. starkeyi to produce lipids for biodiesel synthesis based on the biorefinery concept.
通过酸水解从甘蔗渣（SCB）中提取半纤维素级分，在高压釜和 Parr 反应器中进行评估，旨在将水解物用作 Stachybotrys chartarum 生产脂质的碳源。
在 1.5%（m/v）HSO 和 120°C 下于 Parr 反应器（HH）中处理 20 分钟，可获得最高糖浓度的水解，半纤维素转化率约为 82%。酵母对水解产物的适应提供了良好的发酵性，没有迟滞期。L. starkeyi 发酵半纤维素衍生糖（HH）可得到 27.8%（w/w）的脂质含量和 0.16 g/l.h 的 Y。增加接种量可使脂质含量增加约 61%，达到 44.8%（w/w）。
基于生物炼制概念，从 SCB 获得的半纤维素水解物是生产生物柴油用 L. starkeyi 脂质的潜在底物。
