从甘蔗渣中提取半纤维素水解产物用于生产微生物油脂的斯塔克酵母。
Obtaining hemicellulosic hydrolysate from sugarcane bagasse for microbial oil production by Lipomyces starkeyi.
机构信息
Department of Bioprocess Engineering and Biotechnology, Federal University of Tocantins (UFT), Badejos Street 69-72, Jardim Cervilha, Gurupi, TO, 77404-970, Brazil.
Department of Process Engineering (DEPro), School of Chemical Engineering, State University of Campinas (UNICAMP), Albert Einstein Avenue, 500, Zeferino Vaz University City, Campinas, SP, 13083-852, Brazil.
出版信息
Biotechnol Lett. 2021 May;43(5):967-979. doi: 10.1007/s10529-021-03080-7. Epub 2021 Jan 31.
OBJECTIVE
The extraction of the hemicellulose fraction of sugarcane bagasse (SCB) by acid hydrolysis was evaluated in an autoclave and a Parr reactor aiming the application of the hydrolysate as a carbon source for lipid production by Lipomyces starkeyi.
RESULTS
The hydrolysis that resulted in the highest sugar concentration was obtained by treatment in the Parr reactor (HH) at 1.5% (m/v) HSO and 120 °C for 20 min, reaching a hemicellulose conversion of approximately 82%. The adaptation of the yeast to the hydrolysate provided good fermentability and no lag phase. The fermentation of hemicellulose-derived sugars (HH) by L. starkeyi resulted in a 27.8% (w/w) lipid content and Y of 0.16 g/l.h. Increasing the inoculum size increased the lipid content by approximately 61%, reaching 44.8% (w/w).
CONCLUSION
The hemicellulose hydrolysate from SCB is a potential substrate for L. starkeyi to produce lipids for biodiesel synthesis based on the biorefinery concept.
目的
通过酸水解从甘蔗渣（SCB）中提取半纤维素级分，在高压釜和 Parr 反应器中进行评估，旨在将水解物用作 Stachybotrys chartarum 生产脂质的碳源。
结果
在 1.5%（m/v）HSO 和 120°C 下于 Parr 反应器（HH）中处理 20 分钟，可获得最高糖浓度的水解，半纤维素转化率约为 82%。酵母对水解产物的适应提供了良好的发酵性，没有迟滞期。L. starkeyi 发酵半纤维素衍生糖（HH）可得到 27.8%（w/w）的脂质含量和 0.16 g/l.h 的 Y。增加接种量可使脂质含量增加约 61%，达到 44.8%（w/w）。
结论
基于生物炼制概念，从 SCB 获得的半纤维素水解物是生产生物柴油用 L. starkeyi 脂质的潜在底物。
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