College of Life Science and Technology, Xinjiang University, Urumchi, China.
Antonie Van Leeuwenhoek. 2012 May;101(4):725-31. doi: 10.1007/s10482-011-9686-7. Epub 2011 Dec 29.
A red-pigmented, Gram-negative, strictly aerobic, rod-shaped bacterium which was motile by gliding, designated strain 1351(T), was isolated from the soil of Lengduo, Tibet in China and subjected to a polyphasic taxonomic analysis. The isolate grows optimally at 30°C and pH 7. It grows with NaCl tolerated up to 1.5% (optimum, 0.5%). Phylogenetic analysis based on the 16S rRNA gene sequence shows that strain 1351(T) is closely related to members of the family 'Cytophagaceae' closest sequence similarity was observed with similarity with Adhaeribacter terreus (91.8%). The major whole-cell fatty acids are summed feature 4 (containing anteiso-C(17:1) B and/or iso-C(17:1) I, 29.2%), summed feature 3 (containing C(16:1)ω6c and/or C(16:1)ω7c, 13.0%) and iso-C(15:0) (12.0%). The predominant menaquinone of strain 1351(T) is menaquinone-7 (MK-7) and the G+C content of the DNA is 46.8 mol%. According to the phylogenetic evidence and phenotypic data, strain 1351(T) is considered to represent a new genus and species of the family 'Cytophagaceae' for which the name Rufibacter tibetensis gen nov., sp. nov. is proposed. The type species is R. tibetensis and the type strain is 1351(T) (=CCTCC AB 208084(T) = NRRL B-51285(T)).
一株红色、革兰氏阴性、严格需氧、杆状细菌，可通过滑动方式运动，被命名为 1351(T) 菌株，从中国西藏冷多的土壤中分离得到，并进行了多相分类学分析。该分离株在 30°C 和 pH 7 下生长最佳。它可以在耐受 1.5%（最佳 0.5%）NaCl 的条件下生长。基于 16S rRNA 基因序列的系统发育分析表明，菌株 1351(T) 与“噬细胞科”（Cytophagaceae）的成员密切相关，与 Adhaeribacter terreus 的相似度最高（91.8%）。主要的全细胞脂肪酸是包含 anteiso-C(17:1) B 和/或 iso-C(17:1) I 的总和特征 4（29.2%）、包含 C(16:1)ω6c 和/或 C(16:1)ω7c 的总和特征 3（13.0%）和 iso-C(15:0)（12.0%）。菌株 1351(T) 的主要menaquinone 是menaquinone-7 (MK-7)，DNA 的 G+C 含量为 46.8 mol%。根据系统发育证据和表型数据，菌株 1351(T) 被认为代表了噬细胞科的一个新属和新种，命名为 Rufibacter tibetensis gen nov.，sp. nov.。模式种为 R. tibetensis，模式菌株为 1351(T)（=CCTCC AB 208084(T) = NRRL B-51285(T)）。
