腺相关病毒 Rep 蛋白的 DNA 结合活性对于与单纯疱疹病毒 ICP8 形成依赖于反向末端重复序列的复合物是必需的。
DNA-binding activity of adeno-associated virus Rep is required for inverted terminal repeat-dependent complex formation with herpes simplex virus ICP8.
机构信息
Institute of Virology, Campus Benjamin Franklin, Charité—Medical School, Berlin, Germany.
出版信息
J Virol. 2012 Mar;86(5):2859-63. doi: 10.1128/JVI.06364-11. Epub 2011 Dec 28.
Abstract
Herpes simplex virus (HSV) helper functions for (AAV) replication comprise HSV ICP8 and helicase-primase UL5/UL52/UL8. Here we show that N-terminal amino acids of AAV Rep78 that contact the Rep-binding site within the AAV inverted terminal repeat (ITR) are required for ternary-complex formation with infected-cell protein 8 (ICP8) on AAV single-strand DNA (ssDNA) in vitro and for colocalization in nuclear replication domains in vivo. Our data suggest that HSV-dependent AAV replication is initiated by Rep contacting the AAV ITR and by cooperative binding of ICP8 on AAV ssDNA.
摘要
单纯疱疹病毒 (HSV) 辅助功能 (AAV) 复制包括 HSV ICP8 和解旋酶-引物 UL5/UL52/UL8。在这里，我们表明 AAV Rep78 的 N 端氨基酸与 AAV 反向末端重复 (ITR) 内的 Rep 结合位点接触，这对于 Rep 与感染细胞蛋白 8 (ICP8) 在 AAV 单链 DNA (ssDNA) 上的三元复合物形成以及在体内核复制域中的共定位是必需的。我们的数据表明，HSV 依赖的 AAV 复制是由 Rep 与 AAV ITR 接触以及 ICP8 在 AAV ssDNA 上的协同结合启动的。
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