腺相关病毒2型复制区室的病毒和细胞成分

Vogel Rebecca, Seyffert Michael, Pereira Bruna de Andrade, Fraefel Cornel

Institute of Virology, University of Zurich, Winterthurerstr. 266a, CH-8057 Zurich, Switzerland.

Open Virol J. 2013 Oct 31;7:98-120. doi: 10.2174/1874357901307010098. eCollection 2013.

Adeno-associated virus 2 (AAV2) is a helpervirus-dependent parvovirus with a bi-phasic life cycle comprising latency in absence and lytic replication in presence of a helpervirus, such as adenovirus (Ad) or herpes simplex virus type 1 (HSV-1). Helpervirus-supported AAV2 replication takes place in replication compartments (RCs) in the cell nucleus where virus DNA replication and transcription occur. RCs consist of a defined set of helper virus-, AAV2-, and cellular proteins. Here we compare the profile of cellular proteins recruited into AAV2 RCs or identified in Rep78-associated complexes when either Ad or HSV-1 is the helpervirus, and we discuss the potential roles of some of these proteins in AAV2 and helpervirus infection.

腺相关病毒2型（AAV2）是一种依赖辅助病毒的细小病毒，具有双相生命周期，包括在没有辅助病毒时的潜伏状态以及在存在辅助病毒（如腺病毒（Ad）或单纯疱疹病毒1型（HSV-1））时的裂解复制。辅助病毒支持的AAV2复制发生在细胞核中的复制区室（RCs）中，病毒DNA复制和转录在此处进行。RCs由一组特定的辅助病毒、AAV2和细胞蛋白组成。在这里，我们比较了在Ad或HSV-1作为辅助病毒时，募集到AAV2 RCs中或在Rep78相关复合物中鉴定出的细胞蛋白谱，并讨论了其中一些蛋白在AAV2和辅助病毒感染中的潜在作用。