Department of Biomedical Engineering, School of Science and Engineering, Reykjavik University, Reykjavik, Iceland.
Behav Brain Res. 2012 Apr 1;229(1):10-20. doi: 10.1016/j.bbr.2011.12.025. Epub 2011 Dec 22.
Spontaneous neural activity is instrumental in the formation and maintenance of neural circuits that govern behavior. In mammals, spontaneous activity is observed in the spinal cord, brainstem, diencephalon, and neocortex, and has been most extensively studied in the hippocampus. Using whole-brain in vitro recordings we establish the presence of spontaneous activity in two regions of the zebrafish telenchephalon: the entopeduncular nucleus (EN) and the anterodorsal lobe (ADL). The ADL is part of the lateral telencephalic pallium, an area hypothesized to be functionally equivalent to the mammalian hippocampus. In contrast, the EN has been hypothesized to be equivalent to the mammalian basal ganglia. The observed spontaneous activity is GABA modulated, sensitive to glutamate and chloride transporter antagonists, and is abolished by sodium pump blockers; moreover, the spontaneous activity in the ADL is a slow multiband event (∼100 ms) characterized by an embedded fast ripple wave (∼150-180 Hz). Thus, the spontaneous activity in the ADL shares physiological features of hippocampal sharp waves in rodents. We suggest that this spontaneous activity is important for the formation and maintenance of neural circuits in zebrafish and argue that applying techniques unique to the fish may open novel routes to understand the function of spontaneous activity in mammals.
自发性神经活动对于控制行为的神经回路的形成和维持至关重要。在哺乳动物中，脊髓、脑干、间脑和新皮层中都观察到了自发性活动，并且在海马体中进行了最广泛的研究。使用全脑体外记录，我们在斑马鱼 telecephalon 的两个区域中确定了自发性活动的存在：entopeduncular 核 (EN) 和 anterodorsal 叶 (ADL)。ADL 是外侧端脑脑皮层的一部分，该区域被假设为在功能上与哺乳动物的海马体等效。相比之下，EN 被假设为与哺乳动物的基底节等效。观察到的自发性活动是 GABA 调制的，对谷氨酸和氯离子转运体拮抗剂敏感，并且被钠泵阻滞剂消除；此外，ADL 中的自发性活动是一种缓慢的多波段事件（∼100 ms），其特征是嵌入的快速涟漪波（∼150-180 Hz）。因此，ADL 中的自发性活动具有啮齿动物海马体锐波的生理特征。我们认为这种自发性活动对于斑马鱼神经回路的形成和维持很重要，并认为应用鱼类特有的技术可能会开辟新的途径来理解哺乳动物中自发性活动的功能。
