Institute of Molecular and Cellular Biology, , A*STAR, 61 Biopolis Drive, #08-13 Proteos, Singapore 138673, Singapore.
Philos Trans R Soc Lond B Biol Sci. 2014 Jan 20;369(1637):20120462. doi: 10.1098/rstb.2012.0462. Print 2014 Mar 5.
The rise of zebrafish as a neuroscience research model organism, in conjunction with recent progress in single-cell resolution whole-brain imaging of larval zebrafish, opens a new window of opportunity for research on interval timing. In this article, we review zebrafish neuroanatomy and neuromodulatory systems, with particular focus on identifying homologies between the zebrafish forebrain and the mammalian forebrain. The neuroanatomical and neurochemical basis of interval timing is summarized with emphasis on the potential of using zebrafish to reveal the neural circuits for interval timing. The behavioural repertoire of larval zebrafish is reviewed and we demonstrate that larval zebrafish are capable of expecting a stimulus at a precise time point with minimal training. In conclusion, we propose that interval timing research using zebrafish and whole-brain calcium imaging at single-cell resolution will contribute to our understanding of how timing and time perception originate in the vertebrate brain from the level of single cells to circuits.
斑马鱼作为神经科学研究模式生物的兴起，加上最近在幼鱼斑马鱼的单细胞分辨率全脑成像方面的进展，为间隔计时研究开辟了新的机会。在本文中，我们回顾了斑马鱼的神经解剖学和神经调节系统，特别关注识别斑马鱼前脑与哺乳动物前脑之间的同源性。总结了间隔计时的神经解剖学和神经化学基础，重点介绍了使用斑马鱼揭示间隔计时神经回路的潜力。我们回顾了幼鱼斑马鱼的行为谱，并证明幼鱼斑马鱼只需最少的训练就能准确地预期刺激。总之，我们提出使用斑马鱼和单细胞分辨率的全脑钙成像进行间隔计时研究，将有助于我们理解计时和时间感知如何从单个细胞到回路的水平在脊椎动物大脑中产生。
