School of Biological Sciences, Flinders University, Adelaide, 5001, Australia.
Mol Biol Rep. 2012 May;39(5):5745-54. doi: 10.1007/s11033-011-1384-z. Epub 2011 Dec 30.
The complete mitochondrial genomes of five tiger samples from three subspecies (P. t. sumatrae, P. t. altica, and P. t. tigris) were successfully obtained by using 26 specifically designed Panthera-specific primer sets. The genome organization and gene arrangement of the five tiger samples were similar to each other; however polymorphic tandem repeat sequences were observed in the control region (CR). This led to a difference in the genome lengths obtained from these five samples with an average size of 16,994 bp for the five tiger mitochondrial genomes. The nucleotide base composition was on average as follows: A, 31.8%; T, 27.0%; C, 26.6%; G, 14.6% and exhibited compositional asymmetry. Most of tiger mitochondrial genome characteristics are similar to those of other common vertebrate species; however, some distinctive features were observed in the CR. First, the repetitive sequence 2 (RS 2) contained two repeat units of 80 bp and the first 15 bp of what would be the third repeat motif. The repetitive sequence 3 (RS 3) contained 47-50 repeat motifs of a shorter 8 bp (ACGTAYAC)(n). Second, length heteroplasmy polycystosine (poly-C) stretches was observed at the end of the HV I locus in all tiger samples.
成功地使用 26 个专门设计的 Panthera 特异性引物对从三个亚种（P. t. sumatrae、P. t. altica 和 P. t. tigris）的五个老虎样本中获得了完整的线粒体基因组。五个老虎样本的基因组组织和基因排列彼此相似；然而，在控制区（CR）中观察到了多态性串联重复序列。这导致从这五个样本中获得的基因组长度存在差异，五个老虎线粒体基因组的平均大小为 16994bp。核苷酸碱基组成平均为：A，31.8%；T，27.0%；C，26.6%；G，14.6%，表现出组成不对称性。大多数老虎线粒体基因组特征与其他常见的脊椎动物物种相似；然而，在 CR 中观察到了一些独特的特征。首先，重复序列 2（RS 2）包含两个 80bp 的重复单元，以及第三个重复基序的前 15bp。重复序列 3（RS 3）包含 47-50 个较短的 8bp（ACGTAYAC）（n）重复基序。其次，在所有老虎样本的 HV I 基因座的末端观察到长度异质性多聚胞嘧啶（poly-C）延伸。
