López-Wilchis Ricardo, Del Río-Portilla Miguel Ángel, Guevara-Chumacero Luis Manuel
Departamento de Biología, Universidad Autónoma Metropolitana - Iztapalapa, Av. San Rafael Atlixco No. 186, Col. Vicentina, C.P. 09340, Iztapalapa, México D.F., Mexico.
Departamento de Acuicultura, Centro de Investigación Científica y de Educación Superior de Ensenada, Carretera Ensenada-Tijuana 3918, Zona Playitas, CP 22860, Ensenada, Baja California, Mexico.
Genetica. 2017 Feb;145(1):27-35. doi: 10.1007/s10709-016-9943-9. Epub 2016 Dec 19.
We described the complete mitochondrial genome (mitogenome) of the Wagner's mustached bat, Pteronotus personatus, a species belonging to the family Mormoopidae, and compared it with other published mitogenomes of bats (Chiroptera). The mitogenome of P. personatus was 16,570 bp long and contained a typically conserved structure including 13 protein-coding genes, 22 transfer RNA genes, two ribosomal RNA genes, and one control region (D-loop). Most of the genes were encoded on the H-strand, except for eight tRNA and the ND6 genes. The order of protein-coding and rRNA genes was highly conserved in all mitogenomes. All protein-coding genes started with an ATG codon, except for ND2, ND3, and ND5, which initiated with ATA, and terminated with the typical stop codon TAA/TAG or the codon AGA. Phylogenetic trees constructed using Maximum Parsimony, Maximum Likelihood, and Bayesian inference methods showed an identical topology and indicated the monophyly of different families of bats (Mormoopidae, Phyllostomidae, Vespertilionidae, Rhinolophidae, and Pteropopidae) and the existence of two major clades corresponding to the suborders Yangochiroptera and Yinpterochiroptera. The mitogenome sequence provided here will be useful for further phylogenetic analyses and population genetic studies in mormoopid bats.
我们描述了瓦格纳氏须蝠（Pteronotus personatus）的完整线粒体基因组（线粒体基因组），该物种属于叶口蝠科，并将其与其他已发表的蝙蝠（翼手目）线粒体基因组进行了比较。瓦格纳氏须蝠的线粒体基因组长度为16,570 bp，包含一个典型的保守结构，包括13个蛋白质编码基因、22个转运RNA基因、两个核糖体RNA基因和一个控制区（D环）。除了8个tRNA和ND6基因外，大多数基因都在重链上编码。所有线粒体基因组中蛋白质编码基因和rRNA基因的顺序高度保守。所有蛋白质编码基因均以ATG密码子起始，但ND2、ND3和ND5以ATA起始，并以典型的终止密码子TAA/TAG或密码子AGA终止。使用最大简约法、最大似然法和贝叶斯推断法构建的系统发育树显示出相同的拓扑结构，并表明蝙蝠不同科（叶口蝠科、叶鼻蝠科、蝙蝠科、菊头蝠科和狐蝠科）的单系性以及对应于阳翼手亚目和阴翼手亚目的两个主要分支的存在。这里提供的线粒体基因组序列将有助于叶口蝠科蝙蝠的进一步系统发育分析和群体遗传学研究。
