National Bureau of Fish Genetic Resources, Canal Ring Road, PO Dilkusha, Lucknow, 226 002, UP, India.
Mol Biol Rep. 2012 May;39(5):5921-31. doi: 10.1007/s11033-011-1404-z. Epub 2011 Dec 30.
Clarias batrachus, an Indian catfish species, is endemic to the Indian subcontinent and potential cultivable species. The genomic resources in C. batrachus in the form of ESTs containing microsatellite repeats (EST-SSR) and single nucleotide polymorphisms (SNPs) that are associated with the expressed genes from spleen were mined. From a total of 1,937 ESTs generated, 1,698 unique sequences were obtained, out of which 221 EST-SSRs were identified and 54% could be functionally annotated by similarity searches. A total of 23 contigs containing 3 or more ESTs were found to contain 31 SNP loci, out of which 8 ESTs showed similarity to genes of known function and 1 for hypothetical protein. Nine ESTs with SSRs and/or SNPs identified in this study were reported to be associated with diseases in human and animals. These identified loci can be developed into markers in C. batrachus, which can be useful in linkage mapping, comparative genomics studies and for its genetic improvement programmes.
印度鲶，一种印度鲶鱼物种，是印度次大陆的特有种和潜在的可养殖物种。本研究以 EST-SSR 和 SNP 两种形式从鲶鱼脾脏组织中挖掘了与表达基因相关的基因组资源。在总共生成的 1937 条 EST 中，获得了 1698 条独特的序列，其中鉴定出 221 个 EST-SSR，其中 54%可以通过相似性搜索进行功能注释。总共发现了 23 个包含 3 个或更多 EST 的重叠群，其中包含 31 个 SNP 位点，其中 8 个 EST 与已知功能的基因相似，1 个与假设蛋白相似。本研究中鉴定的 9 个含有 SSR 和/或 SNP 的 EST 被报道与人类和动物的疾病有关。这些鉴定的基因座可以开发为鲶鱼的标记，可用于连锁图谱、比较基因组学研究和遗传改良计划。
