Department of Cardiology, Sanjay Gandhi PGIMS, Lucknow, Uttar Pradesh, India.
Mol Biol Rep. 2012 May;39(5):5995-6000. doi: 10.1007/s11033-011-1412-z. Epub 2011 Dec 30.
Nitric Oxide (NO) is an important molecule carrying number of different functions in humans. Published studies suggest that it may inhibit several key steps involved in the pathogenesis of atherosclerosis. Inhibition or reduction of NO due to Glu298Asp polymorphism may accelerate atherosclerosis. The aim of this study was to determine whether Glu298Asp polymorphism is implicated in the pathogenesis of coronary artery disease (CAD) among North Indian population from the state of Uttar Pradesh, India. We selected 253 CAD patients and 174 healthy, normotensive, non-diabetic controls, which were matched for gender and ethnicity. The Glu298Asp (rs1799983) variant was detected by genotyping subjects, using a polymerase chain reaction followed by restriction fragment length polymorphism. There was no significant difference found in the genotypic and allelic frequencies between patients and controls. Our study indicated that Glu298Asp polymorphism does not play any critical role in the pathogenesis of CAD, at least in North Indian population.
一氧化氮（NO）是一种在人体中具有多种不同功能的重要分子。已发表的研究表明，它可能抑制动脉粥样硬化发病机制中的几个关键步骤。由于 Glu298Asp 多态性导致的 NO 抑制或减少可能会加速动脉粥样硬化的形成。本研究旨在确定 Glu298Asp 多态性是否与印度北方邦北方印度人群的冠状动脉疾病（CAD）发病机制有关。我们选择了 253 名 CAD 患者和 174 名健康、血压正常、非糖尿病对照者，这些对照者在性别和种族方面相匹配。使用聚合酶链反应（PCR）后限制性片段长度多态性，对受试者的 Glu298Asp(rs1799983) 变体进行基因分型。在患者和对照组之间，基因型和等位基因频率没有发现显著差异。我们的研究表明，Glu298Asp 多态性在 CAD 的发病机制中至少在北方印度人群中不起关键作用。
