Luna Rosa, Rondón Ana G, Aguilera Andrés
Centro Andaluz de Biología Molecular y Medicina Regenerativa CABIMER, Universidad de Sevilla-CSIC, Av Américo Vespucio s/n, 41092 Sevilla, Spain. rlvarp@is/es
Biochim Biophys Acta. 2012 Jun;1819(6):514-20. doi: 10.1016/j.bbagrm.2011.11.012. Epub 2011 Dec 20.
Coupling of transcription with mRNA processing and export has been shown to be relevant to efficient gene expression. A number of studies have determined that THO/TREX, a nuclear protein complex conserved from yeast to humans, plays an important role in mRNP biogenesis connecting transcription elongation, mRNA export and preventing genetic instability. Recent data indicates that THO could be relevant to different mRNA processing steps, including the 3'-end formation, transcript release and export. Novel connections of THO to proteins related to the splicing machinery, provide new views about possible functions of THO in mRNP biogenesis. In this review, we summarize the previous and new results concerning the impact of THO in transcription and its biological implications, with a special emphasis on the relationship with THSC/TREX-2 and other functionally related factors involved in mRNA biogenesis and export. The emerging picture presents THO as a dynamic complex interacting with the nascent RNA and with different factors connecting nuclear functions necessary for mRNP biogenesis with genome integrity, cellular homeostasis and development. This article is part of a Special Issue entitled: Nuclear Transport and RNA Processing.
转录与mRNA加工及输出的偶联已被证明与高效基因表达相关。多项研究已确定，THO/TREX是一种从酵母到人类都保守的核蛋白复合体，在连接转录延伸、mRNA输出及防止遗传不稳定的mRNP生物合成中起重要作用。最新数据表明，THO可能与不同的mRNA加工步骤相关，包括3'端形成、转录本释放及输出。THO与剪接机制相关蛋白的新联系，为THO在mRNP生物合成中的可能功能提供了新观点。在本综述中，我们总结了关于THO在转录中的影响及其生物学意义的先前和新结果，特别强调了与THSC/TREX-2以及参与mRNA生物合成和输出的其他功能相关因子的关系。新出现的情况表明，THO是一个动态复合体，与新生RNA以及将mRNP生物合成所需的核功能与基因组完整性、细胞内稳态和发育联系起来的不同因子相互作用。本文是名为“核运输与RNA加工”的特刊的一部分。
