Wang Xian-Lei, Gao Xing-Wang, Li Guan, Wang Hui-Lin, Geng Shou-Dong, Kang Feng, Nie Xiang-Xiang
Life Science and Technology Department, Xinjiang University, Urumchi, China.
Yi Chuan. 2011 Dec;33(12):1398-408. doi: 10.3724/sp.j.1005.2011.01398.
A genetic map of melon was constructed using 143 F2 population developed from a cross between two distant lines Ano2 of Japan and Hami melon K413. The map contains 12 linkage groups and 142 markers, including 121 AFLPs, 16 SSRs, 3 STSs, 2 trait markers and covers 1 014.2 cM. Composite interval mapping (CIM) method was used to detect QTLs involved in melon fruit and seed traits: fruit length (FL), fruit width (FW), fruit shape (length/width, FS), centre sugar (CS), edge sugar (ES), flesh texture (FT), seed length (SL), seed width (SW), seed shape (SS), and seed weight (SW). The result showed that Flesh was located between AFLP markers NDAA and NCFA on C9. A total of 25 QTLs were detected for other traits and some QTLs were co-located with each other. The QTLs Sl5.1, Sw5.1, and Swt5.1 located on linkage C5 between NCA and N73C explained a significant portion of associated phenotypic variation (R2=17%, 19%, 23%). The allele from Ano2 obviously suppressed the length, width, and weight of melon seed; the QTLs between N73A and NFDA on C8 were involved in seed width, shape, and weight; the QTL Fs8.1 on C8 was detected using both F2 and F3 fruit data and explained a significant portion of phenotypic variation 25% and 19%. Fs8.1 showed partly dominant, and the allele from Ano2 sup-pressed elongation of fruit to form round melon. The QTLs related to centre sugar, edge sugar, and fruit texture were also detected in this research.
利用从日本远缘品系Ano2与哈密瓜K413杂交得到的143个F2群体构建了甜瓜遗传图谱。该图谱包含12个连锁群和142个标记，其中包括121个AFLP标记、16个SSR标记、3个STS标记、2个性状标记，覆盖1014.2厘摩。采用复合区间作图（CIM）方法检测甜瓜果实和种子性状相关的QTL，这些性状包括果实长度（FL）、果实宽度（FW）、果形（长宽比，FS）、中心糖含量（CS）、边缘糖含量（ES）、果肉质地（FT）、种子长度（SL）、种子宽度（SW）、种子形状（SS）和种子重量（SW）。结果表明，果肉质地位于C9连锁群上的AFLP标记NDAA和NCFA之间。共检测到25个与其他性状相关的QTL，部分QTL位于同一位置。位于C5连锁群上NCA与N73C之间的QTL Sl5.1、Sw5.1和Swt5.1解释了相当一部分相关表型变异（R2分别为17%、19%、23%）。来自Ano2的等位基因明显抑制了甜瓜种子的长度、宽度和重量；C8连锁群上N73A与NFDA之间的QTL与种子宽度、形状和重量有关；利用F2和F3果实数据均检测到C8连锁群上的QTL Fs8.1，该QTL解释了25%和19%的表型变异。Fs8.1表现出部分显性，来自Ano2的等位基因抑制果实伸长形成圆形甜瓜。本研究还检测到了与中心糖、边缘糖和果实质地相关的QTL。
