Department of Vegetable Research, Agricultural Research Organization, Newe Ya'ar Research Center, P.O. Box 1021, Ramat Yishay, 30095, Israel.
Theor Appl Genet. 2010 Aug;121(3):511-33. doi: 10.1007/s00122-010-1327-4. Epub 2010 Apr 17.
A genetic map of melon enriched for fruit traits was constructed, using a recombinant inbred (RI) population developed from a cross between representatives of the two subspecies of Cucumis melo L.: PI 414723 (subspecies agrestis) and 'Dulce' (subspecies melo). Phenotyping of 99 RI lines was conducted over three seasons in two locations in Israel and the US. The map includes 668 DNA markers (386 SSRs, 76 SNPs, six INDELs and 200 AFLPs), of which 160 were newly developed from fruit ESTs. These ESTs include candidate genes encoding for enzymes of sugar and carotenoid metabolic pathways that were cloned from melon cDNA or identified through mining of the International Cucurbit Genomics Initiative database (http://www.icugi.org/). The map covers 1,222 cM with an average of 2.672 cM between markers. In addition, a skeleton physical map was initiated and 29 melon BACs harboring fruit ESTs were localized to the 12 linkage groups of the map. Altogether, 44 fruit QTLs were identified: 25 confirming QTLs described using other populations and 19 newly described QTLs. The map includes QTLs for fruit sugar content, particularly sucrose, the major sugar affecting sweetness in melon fruit. Six QTLs interacting in an additive manner account for nearly all the difference in sugar content between the two genotypes. Three QTLs for fruit flesh color and carotenoid content were identified. Interestingly, no clear colocalization of QTLs for either sugar or carotenoid content was observed with over 40 genes encoding for enzymes involved in their metabolism. The RI population described here provides a useful resource for further genomics and metabolomics studies in melon, as well as useful markers for breeding for fruit quality.
构建了一个富含果实性状的甜瓜遗传图谱，该图谱使用了 PI 414723（亚种 agrestis）和 'Dulce'（亚种 melo）代表之间杂交的重组自交系（RI）群体开发。在以色列和美国的两个地点的三个季节对 99 个 RI 系进行了表型分析。该图谱包含 668 个 DNA 标记（386 SSR、76 SNPs、6 INDEL 和 200 AFLP），其中 160 个是从果实 EST 中开发的新标记。这些 EST 包括编码糖和类胡萝卜素代谢途径的候选基因，这些基因是从甜瓜 cDNA 中克隆的，或者是通过挖掘国际葫芦科基因组计划数据库（http://www.icugi.org/）鉴定的。图谱覆盖 1222cM，标记之间的平均距离为 2.672cM。此外，启动了骨架物理图谱，并将 29 个含有果实 EST 的甜瓜 BAC 定位到图谱的 12 个连锁群上。总共鉴定了 44 个果实 QTL：25 个确认 QTL 是使用其他群体描述的，19 个是新描述的 QTL。该图谱包括果实含糖量的 QTL，特别是蔗糖，这是影响甜瓜果实甜度的主要糖分。6 个以累加方式相互作用的 QTL 几乎解释了两个基因型之间糖含量的所有差异。鉴定出了 3 个果实果肉颜色和类胡萝卜素含量的 QTL。有趣的是，没有观察到与糖或类胡萝卜素含量相关的 QTL 与参与其代谢的 40 多个基因明显共定位。本文描述的 RI 群体为甜瓜的进一步基因组学和代谢组学研究提供了有用的资源，也为果实品质的育种提供了有用的标记。
