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关于弱氧化醋杆菌将山梨醇转化为山梨糖的研究(作者译)

[Researches to the conversion of sorbit into sorbose by Acetobacter suboxydans (author's transl)].

作者信息

Kölblin R, Tröger R

出版信息

Zentralbl Bakteriol Parasitenkd Infektionskr Hyg. 1977;132(3):196-203.

PMID:22208
Abstract

The production of sorbose by Acetobacter suboxydans (4) is closely related to the concentration of sorbit in the medium. An increasing concentration of sorbit gives rise to the inhibition of cell reproduction; followed by a decrease of sorbose content in the culture medium. The decrease of sorbose yield in concentrations of about 15% sorbit in medium indicates the decreasing metabolism rate of the total population of Acetobacter suboxydans (4) culture and does not refer to the ability of the individual bacterium cell to produce sorbose. Relevant research work showed, that sorbose production for each bacterium cell distinctly increased with the decrease of the number of cells in a population of Acetobacter suboxydans (4) as a consequence of the application of an increased sorbit concentration. An unrestrained reproduction of bacteria could be obtained by exluding all factors involved in the contamination of sorbit and exhibiting toxic effects. Therefore the organisms could be offered a greater concentration of sorbit for conversion into sorbose. Thus sorbose yield would be increased, respectively. The total conversion of the C-source into sorbose could not be obtained with Acetobacter ruboxydans (4).

摘要

弱氧化醋杆菌(4)生产山梨糖的过程与培养基中山梨醇的浓度密切相关。山梨醇浓度的增加会抑制细胞繁殖,随后培养基中山梨糖的含量会降低。当培养基中山梨醇浓度约为15%时山梨糖产量的下降表明弱氧化醋杆菌(4)培养物总体的代谢速率降低,而不是指单个细菌细胞生产山梨糖的能力。相关研究工作表明,由于山梨醇浓度增加,随着弱氧化醋杆菌(4)群体中细胞数量的减少,每个细菌细胞的山梨糖产量明显增加。通过排除所有与山梨醇污染有关且具有毒性作用的因素,可以实现细菌的无限制繁殖。因此,可以为微生物提供更高浓度的山梨醇以转化为山梨糖。这样山梨糖产量将相应增加。氧化醋杆菌(4)无法将碳源完全转化为山梨糖。

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Kinetic studies on sorbose fermentation.山梨糖发酵的动力学研究。
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