van der Bosch J, Sommer I, Maier H, Rahmig W
Z Naturforsch C Biosci. 1979 Mar-Apr;34(3-4):279-83.
Lowered extracellular [Ca2+] cause low growth rates and low stationary cell densities in 3T3 cell cultures as compared to physiological [Ca2%]. Under otherwise constant conditions the extracellular [Ca2+] determines a stable stationary cell density, which can be reached by increase of net cell number or decrease of net cell number, depending on cell density at the time of [Ca2+] adjustment. SV40-3T3 cells do not show this [Ca2+] dependency. At 39 degrees C 3T3 and SV40-3T3 cell populations show an increased growth rate at low cell densities as compared to cell populations at 35 degrees C. Approaching the stationary density the growth rate of both cell sorts is reduced faster at 39 degrees C than at 35 degrees C, leading to lower stationary cell densities at 39 degrees C than at 35 degrees C. A temperature change from 39 degrees C to 35 degrees C or in the opposite direction can affect the stationary cell density of 3T3 cell populations only if applied before reduction of growth rate by density-dependent growth-inhibiting principles has taken place.
与生理[Ca2+]相比，细胞外[Ca2+]降低会导致3T3细胞培养物中的生长速率降低和静止细胞密度降低。在其他条件恒定的情况下，细胞外[Ca2+]决定了一个稳定的静止细胞密度，根据[Ca2+]调整时的细胞密度，可通过增加净细胞数或减少净细胞数来达到该密度。SV40 - 3T3细胞不表现出这种[Ca2+]依赖性。在39℃时，与35℃时的细胞群体相比，3T3和SV40 - 3T3细胞群体在低细胞密度下显示出更高的生长速率。接近静止密度时，两种细胞类型在39℃时的生长速率比在35℃时更快地降低，导致39℃时的静止细胞密度低于35℃时。只有在密度依赖性生长抑制原理导致生长速率降低之前施加，从39℃到35℃或相反方向的温度变化才会影响3T3细胞群体的静止细胞密度。
