Adam G, Schumann C
Cell Biophys. 1981 Sep;3(3):189-209. doi: 10.1007/BF02782623.
Interfacial properties of the outer cell membrane of normal and transformed in vitro cultures of mouse 3T3 cells have been investigated. The contact angles of sessile drops on dried cell preparations were measured and the interfacial tensions derived using the thermodynamic approach introduced by Neumann. Interfacial tensions were found to be within an order of magnitude of those determined for other cell and model membranes. Treatment of cells with calf serum, a stimulant to proliferation, resulted in a decrease in the interfacial tension of normal and transformed cells, whereas use of concanavalin A and its succinylated derivative lead to an increase of interfacial tensions of both cell types. These and further results show a detailed correlation between the growth-regulating effects and the effects on interfacial properties of these proliferation-modifying factors. An interpretation of the results of serum depression of the interfacial tension in terms of a binding equilibrium dependent on the concentration of humoral growth factors in the medium is attempted.
对小鼠3T3细胞正常及体外转化培养物的外细胞膜界面特性进行了研究。测量了在干燥细胞制剂上静滴的接触角，并使用诺伊曼引入的热力学方法得出界面张力。发现界面张力与其他细胞和模型膜所测定的界面张力在一个数量级范围内。用小牛血清（一种增殖刺激剂）处理细胞，导致正常细胞和转化细胞的界面张力降低，而使用伴刀豆球蛋白A及其琥珀酰化衍生物则导致两种细胞类型的界面张力增加。这些以及进一步的结果表明，这些增殖调节因子的生长调节作用与对界面特性的影响之间存在详细的相关性。尝试根据依赖于培养基中体液生长因子浓度的结合平衡来解释血清降低界面张力的结果。
