Comanescu Maria, Annaratone Laura, D'Armento Giuseppe, Cardos Georgeta, Sapino Anna, Bussolati Gianni
Victor Babes Institute, Bucharest, Romania, Spl. Independentei 99-101, 050096 Bucharest - Romania.
Recent Pat DNA Gene Seq. 2012 Apr;6(1):22-32. doi: 10.2174/187221512799303190.
Histopathological diagnosis using Formalin-Fixed Paraffin Embedded (FFPE) tissues is essential for the prognostic and therapeutic management of cancer patients. Pathologists are being confronted with increasing demands, from both clinicians and patients, to provide immunophenotypic and gene expression data from FFPE tissues to allow the planning of personalized therapeutic regimens. Recent improvements in the protocols for pre-analysis processing of pathological tissues aim to better preserve cellular details and to conserve antigens and nucleic acid sequences. These developments have been recently patented. The international protocol for the transporting of surgical specimens from the surgical theatre to the pathology department is to immerse the specimen in formalin. The alternative method of sealing the specimens into bags under a vacuum and then cooling is a well-accepted and environmentally safe procedure that overcomes the many drawbacks linked to transfer in formalin. Importantly, RNA is notoriously poorly preserved in FFPE tissue. Due to this, successful procedures for the extraction of genetic information from archival tissues have been the object of several studies and patents. Novel molecular approaches for RT-qPCR and gene array analysis on FFPE tissues are presented here. Moreover, a major advance is reported in this study, the observation that tissue fixation in cold conditions allows a much better preservation of nucleic acid sequences.
使用福尔马林固定石蜡包埋（FFPE）组织进行组织病理学诊断对于癌症患者的预后和治疗管理至关重要。临床医生和患者对病理学家的要求越来越高，希望他们能提供FFPE组织的免疫表型和基因表达数据，以便制定个性化的治疗方案。病理组织分析前处理方案的最新改进旨在更好地保留细胞细节，并保存抗原和核酸序列。这些进展最近已获得专利。将手术标本从手术室运送到病理科的国际规程是将标本浸入福尔马林中。另一种方法是将标本真空密封在袋子中，然后冷却，这是一种公认的、对环境安全的程序，克服了与福尔马林转运相关的许多缺点。重要的是，众所周知，FFPE组织中的RNA保存不佳。因此，从存档组织中提取遗传信息的成功程序一直是多项研究和专利的主题。本文介绍了用于FFPE组织RT-qPCR和基因阵列分析的新型分子方法。此外，本研究报告了一项重大进展，即观察到在低温条件下进行组织固定可以更好地保存核酸序列。
