Key Laboratory of Technology on Electrochemical Energy Storage and Power Generation in Guangdong Universities, School of Chemistry and Environment, South China Normal University, Guangzhou, PR China.
Spectrochim Acta A Mol Biomol Spectrosc. 2012 Mar;88:124-9. doi: 10.1016/j.saa.2011.12.015. Epub 2011 Dec 16.
The quenching and recovery kinetics of photoluminescence of Ru(bpy)(2)(tatp) (Ru1) and Ru(bpy)(2)(dmtatp) (Ru2) intercalated within DNA (where bpy=2,2'-bipyridine, tatp=1,4,8,9-tetra-aza-triphenylene and dmtatp=2,3-dimethyl-1,4,8,9-tetra-aza-triphenylene) have been investigated by steady-state and time-resolved methods performed at various temperatures (293-333K). Two complexes Ru1 and Ru2 show a single-exponential luminescence decay with τ(Ru1)=246.0 ns and τ(Ru2)=513.5 ns, whose luminescence upon intercalating into DNA exhibits very consistent bi-exponential decay changes. The addition of Cu(2+) ions is found to dynamically quench the luminescence of both DNA-bound Ru(II) complexes, involving a spontaneous exothermic process. The sequential addition of EDTA can partially recover the luminescence quenched by Cu(2+), however depending on methyl substituents of the intercalative ligand. The chemical conversion and luminescence control mechanism of the two DNA-bound Ru(II) complexes is discussed in detail. The present results should be of value for better understanding chemical modulation of DNA-bound Ru(II) complexes as luminescence probes.
Ru(bpy)(2)(tatp)（Ru1）和Ru(bpy)(2)(dmtatp)（Ru2）插层在 DNA 内的光致发光的猝灭和恢复动力学已通过在不同温度（293-333 K）下进行的稳态和时间分辨方法进行了研究。两个配合物 Ru1 和 Ru2 表现出单指数荧光衰减，τ（Ru1）=246.0 ns 和 τ（Ru2）=513.5 ns，其在插入 DNA 后表现出非常一致的双指数衰减变化。发现添加 Cu（2+）离子会动态猝灭两种结合在 DNA 上的 Ru（II）配合物的发光，涉及自发的放热过程。顺序添加 EDTA 可以部分恢复 Cu（2+）猝灭的发光，但取决于插入配体的甲基取代基。详细讨论了两种结合在 DNA 上的 Ru（II）配合物的化学转化和发光控制机制。这些结果对于更好地理解作为发光探针的结合在 DNA 上的 Ru（II）配合物的化学调节应该是有价值的。
