Key Lab of Technology on Electrochemical Energy Storage and Power Generation in Guangdong Universities, School of Chemistry and Environment, South China Normal University, Guangzhou 510006, PR China.
Spectrochim Acta A Mol Biomol Spectrosc. 2012 Nov;97:297-305. doi: 10.1016/j.saa.2012.06.016. Epub 2012 Jun 17.
An imidazophenanthroline-containing ruthenium(II) complex Ru(bpy)(2)(mbpibH2) (bpy=2,2'-bipyridine, mbpibH2=1,3-bis([1,10]phenanthroline[5,6-d]imidazol-2-yl)benzene) can bind DNA through groove-binding and/or non-classical intercalation modes, revealed by spectrophotometric methods, viscosity measurements and variable ionic strength experiments. On the basis of binding interactions between cationic Ru(bpy)(2)(mbpibH2) and anionic DNA at a molar ratio of 1:1, a yellow transparent cast film has been assembled on an indium-tin oxide (ITO) surface using a solution-based self-standing method. The prepared DNA-Ru(bpy)(2)(mbpibH2) film shows a bi-exponential luminescence decay with τ(1)=62.1 ns (8.0%) and τ(2)=594.5 ns (92.0%), whose lifetimes become much shorter than those of DNA-bound Ru(bpy)(2)(mbpibH2) in buffer solutions. The Ru(II) complex with a free bi-dentate coordination site in the DNA cast film shows tunable luminescence, quenched dynamically by Cu(2+) and restored by using EDTA to eliminate two modes of Cu(2+)-binding. The results from this study provide a significant foundation for better understanding the fabrication and modulation of a DNA-based solid luminescence device using the Ru(II) complexes as DNA-concentrating and signal-sensing agents.
一种含咪唑并菲咯啉的钌（II）配合物Ru(bpy)(2)(mbpibH2)（bpy=2,2'-联吡啶，mbpibH2=1,3-双([1,10]菲咯啉[5,6-d]咪唑-2-基)苯）可以通过光谱法、粘度测量和可变离子强度实验表明，通过沟结合和/或非经典嵌入模式结合 DNA。基于阳离子Ru(bpy)(2)(mbpibH2)与阴离子 DNA 之间的结合相互作用，在摩尔比为 1:1 的情况下，使用基于溶液的自支撑方法在铟锡氧化物（ITO）表面组装了黄色透明的铸造膜。制备的 DNA-Ru(bpy)(2)(mbpibH2)膜表现出双指数荧光衰减，τ(1)=62.1 ns（8.0%）和τ(2)=594.5 ns（92.0%），其寿命比缓冲溶液中 DNA 结合的Ru(bpy)(2)(mbpibH2)短得多。在 DNA 铸造膜中具有游离双齿配位位的 Ru(II)配合物表现出可调谐的发光，被 Cu(2+)动态猝灭，并通过使用 EDTA 消除两种 Cu(2+)结合模式来恢复。这项研究的结果为更好地理解使用 Ru(II)配合物作为 DNA 浓缩和信号传感剂的基于 DNA 的固态发光器件的制造和调制提供了重要基础。
