Shanghai Key Laboratory of Veterinary Biotechnology, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China.
Arch Virol. 2012 Mar;157(3):573-8. doi: 10.1007/s00705-011-1205-7. Epub 2011 Dec 31.
Porcine kobuvirus was first identified in 2007 in Hungary. The virus has been detected in several Asian countries. In our study, the complete genome of the recently identified porcine kobuvirus strain SH-W-CHN was amplified by RT-PCR and was sequenced. Dendrograms indicated that SH-W-CHN is closely related to other porcine kobuviruses. To identify sites of possible recombination within the genome of the SH-W-CHN strain, the SimPlot program was used to perform recombination analysis. The results showed that no significant recombination event between strain S-1-HUN and Y-1-CHI had occurred. However, certain possible recombination signals were identified, indicating that some early recombination events may have contributed to the genome of SH-W-CHN. This study further confirmed the existence of multiple lineages of porcine kobuvirus and indicated that homologous recombination may be a driving force in its evolution.
猪博卡病毒于 2007 年在匈牙利首次被发现。该病毒已在多个亚洲国家被检测到。在本研究中，通过 RT-PCR 扩增并测序了最近鉴定的猪博卡病毒株 SH-W-CHN 的全基因组。系统发育树分析表明，SH-W-CHN 与其他猪博卡病毒密切相关。为了鉴定 SH-W-CHN 株基因组中可能的重组部位，使用 SimPlot 程序进行重组分析。结果表明，S-1-HUN 株和 Y-1-CHI 株之间没有发生明显的重组事件。然而，发现了某些可能的重组信号，表明早期的重组事件可能对 SH-W-CHN 的基因组有一定的贡献。本研究进一步证实了猪博卡病毒存在多个谱系，并且同源重组可能是其进化的驱动力之一。
