Division of Ruminant Medicine and Veterinary Epidemiology, Department of Clinical Science, Swedish University of Agricultural Sciences, Box 7019, 750 07, Uppsala, Sweden.
Arch Virol. 2012 Apr;157(4):601-7. doi: 10.1007/s00705-011-1209-3. Epub 2011 Dec 31.
Bovine respiratory syncytial virus (BRSV) is one of the major causes of bovine respiratory disease worldwide. In order to study the molecular epidemiology of the virus, samples from 30 BRSV outbreaks in cattle herds located in different parts of Sweden were collected from 2007 to 2011. The samples were analyzed by PCR, and the glycoprotein (G) gene was sequenced. BRSV was detected in outbreaks of respiratory disease in both dairy and feedlot herds most often during the winter period but also during the summer months (May to August). This indicates that circulation of the virus between herds occurs throughout the year. Comparative sequence analysis revealed a high degree (more than 94.5%) of sequence identity among the collected strains. Phylogenetic analysis showed that 29 out of the 30 strains formed a unique clade. Identical sequences found in herds sampled within a few months' time suggested that these herds were part of a common transmission chain. One strain from a single outbreak in a herd in southern Sweden clustered with Danish strains and showed a distant relationship to the rest of the Swedish strains. Further studies are highly warranted to clarify the inter-herd transmission routes of BRSV. Such knowledge is essential for the control of the spread of this virus between herds, regions and even countries.
牛呼吸道合胞体病毒（BRSV）是世界范围内引起牛呼吸道疾病的主要原因之一。为了研究该病毒的分子流行病学，我们于 2007 年至 2011 年期间，从瑞典不同地区的 30 个牛群暴发的呼吸道疾病中采集了样本。通过 PCR 分析和糖蛋白（G）基因测序对这些样本进行了检测。结果表明，BRSV 在冬季和夏季（5 月至 8 月）的牛群中均会引起呼吸道疾病的暴发。这表明该病毒在牛群之间全年均有传播。序列比较分析显示，所收集的病毒株之间具有高度的序列同一性（超过 94.5%）。系统发育分析显示，30 个分离株中的 29 个形成了一个独特的分支。在几个月内采集的牛群中发现的相同序列表明，这些牛群属于同一传播链。来自瑞典南部一个牛群的单一暴发中的一株与丹麦株聚类，与其余瑞典株的亲缘关系较远。需要进一步的研究来阐明 BRSV 在牛群之间的传播途径。这种知识对于控制该病毒在牛群、地区甚至国家之间的传播至关重要。
