刚地弓形虫 Sis1 样 J 结构域蛋白是一种胞质伴侣，与 HSP90/HSP70 复合物相关。
Toxoplasma gondii Sis1-like J-domain protein is a cytosolic chaperone associated to HSP90/HSP70 complex.
机构信息
Laboratorio de Parasitología Molecular, IIB-INTECH, CONICET-UNSAM, Av. Intendente Marino Km. 8.2, C.C 164, (B7130IIWA), Chascomús, Prov. Buenos Aires, Argentina.
出版信息
Int J Biol Macromol. 2012 Apr 1;50(3):725-33. doi: 10.1016/j.ijbiomac.2011.12.012. Epub 2011 Dec 23.
Abstract
Toxoplasma gondii is an obligate intracellular protozoan parasite in which 36 predicted Hsp40 family members were identified by searching the T. gondii genome. The predicted protein sequence from the gene ID TGME49_065310 showed an amino acid sequence and domain structure similar to Saccharomyces cerevisiae Sis1. TgSis1 did not show differences in its expression profile during alkaline stress by microarray analysis. Furthermore, TgSis1 showed to be a cytosolic Hsp40 which co-immunoprecipitated with T. gondii Hsp70 and Hsp90. Structural modeling of the TgSis1 peptide binding fragment revealed structural and electrostatic properties different from the experimental model of human Sis1-like protein (Hdj1). Based on these differences; we propose that TgSis1 may be a potentially attractive drug target for developing a novel anti-T. gondii therapy.
摘要
刚地弓形虫是一种专性细胞内原生动物寄生虫，通过搜索刚地弓形虫基因组，鉴定出 36 种预测的 HSP40 家族成员。从基因 ID TGME49_065310 预测的蛋白质序列显示出与酿酒酵母 Sis1 相似的氨基酸序列和结构域结构。通过微阵列分析，TgSis1 在碱性应激过程中的表达谱没有差异。此外，TgSis1 显示为一种胞质 HSP40，与刚地弓形虫的 HSP70 和 HSP90 共免疫沉淀。TgSis1 肽结合片段的结构建模揭示了与人类 Sis1 样蛋白（Hdj1）实验模型不同的结构和静电特性。基于这些差异，我们提出 TgSis1 可能是一种有吸引力的药物靶点，可用于开发新型抗刚地弓形虫治疗方法。
相似文献
1
Toxoplasma gondii Sis1-like J-domain protein is a cytosolic chaperone associated to HSP90/HSP70 complex.刚地弓形虫 Sis1 样 J 结构域蛋白是一种胞质伴侣，与 HSP90/HSP70 复合物相关。
Int J Biol Macromol. 2012 Apr 1;50(3):725-33. doi: 10.1016/j.ijbiomac.2011.12.012. Epub 2011 Dec 23.
2
The Hsp90 co-chaperone p23 of Toxoplasma gondii: Identification, functional analysis and dynamic interactome determination.刚地弓形虫的热休克蛋白90共伴侣蛋白p23：鉴定、功能分析及动态相互作用组测定
Mol Biochem Parasitol. 2010 Aug;172(2):129-40. doi: 10.1016/j.molbiopara.2010.04.004. Epub 2010 Apr 18.
3
Heat shock protein 40-Gok1 isolation from Toxoplasma gondii RH strain.从刚地弓形虫RH株中分离热休克蛋白40-Gok1
Protein Pept Lett. 2013 Dec;20(12):1294-301. doi: 10.2174/092986652012131112113608.
4
Roles of intramolecular and intermolecular interactions in functional regulation of the Hsp70 J-protein co-chaperone Sis1.分子内和分子间相互作用在热休克蛋白70（Hsp70）J蛋白共伴侣Sis1功能调控中的作用
J Mol Biol. 2015 Apr 10;427(7):1632-43. doi: 10.1016/j.jmb.2015.02.007. Epub 2015 Feb 14.
5
Cytosolic and ER J-domains of mammalian and parasitic origin can functionally interact with DnaK.源自哺乳动物和寄生虫的胞质和内质网J结构域可与DnaK发生功能性相互作用。
Int J Biochem Cell Biol. 2007;39(4):736-51. doi: 10.1016/j.biocel.2006.11.006. Epub 2006 Nov 23.
6
The Type II Hsp40 Sis1 cooperates with Hsp70 and the E3 ligase Ubr1 to promote degradation of terminally misfolded cytosolic protein.Sis1 是一种 II 型 HSP40，它与 HSP70 和 E3 连接酶 Ubr1 合作，促进细胞溶质中末端错误折叠的蛋白质的降解。
PLoS One. 2013;8(1):e52099. doi: 10.1371/journal.pone.0052099. Epub 2013 Jan 16.
7
Direct interactions between molecular chaperones heat-shock protein (Hsp) 70 and Hsp40: yeast Hsp70 Ssa1 binds the extreme C-terminal region of yeast Hsp40 Sis1.分子伴侣热休克蛋白（Hsp）70与Hsp40之间的直接相互作用：酵母Hsp70 Ssa1与酵母Hsp40 Sis1的极端C末端区域结合。
Biochem J. 2002 Jan 1;361(Pt 1):27-34. doi: 10.1042/0264-6021:3610027.
8
Toxoplasma gondii Hsp90: potential roles in essential cellular processes of the parasite.刚地弓形虫热休克蛋白90：在该寄生虫基本细胞过程中的潜在作用
Parasitology. 2014 Aug;141(9):1138-47. doi: 10.1017/S0031182014000055. Epub 2014 Feb 21.
9
The chloroplast DnaJ homolog CDJ1 of Chlamydomonas reinhardtii is part of a multichaperone complex containing HSP70B, CGE1, and HSP90C.莱茵衣藻的叶绿体DnaJ同源物CDJ1是一个包含HSP70B、CGE1和HSP90C的多分子伴侣复合体的一部分。
Plant Physiol. 2008 Dec;148(4):2070-82. doi: 10.1104/pp.108.127944. Epub 2008 Oct 17.
10
The crystal structure of the putative peptide-binding fragment from the human Hsp40 protein Hdj1.来自人类热休克蛋白40（Hsp40）蛋白Hdj1的假定肽结合片段的晶体结构。
BMC Struct Biol. 2008 Jan 22;8:3. doi: 10.1186/1472-6807-8-3.
引用本文的文献
1
Biochemistry of Heat Shock Proteins From Human Intracellular Protozoan Parasites as Diagnostic and Therapeutic Biomarkers.来自人类细胞内原生动物寄生虫的热休克蛋白的生物化学作为诊断和治疗生物标志物
Biochemistry. 2025 Jun 17;64(12):2529-2543. doi: 10.1021/acs.biochem.5c00120. Epub 2025 Jun 2.
2
Toxoplasma gondii bradyzoite-specific BAG1 is nonessential for cyst formation due to compensation by other heat-shock proteins.刚地弓形虫缓殖子特异性 BAG1 由于其他热休克蛋白的补偿而对囊形成不是必需的。
Parasit Vectors. 2024 Jul 30;17(1):322. doi: 10.1186/s13071-024-06339-w.
3
Analysis of the Interactome of the Tgj1 HSP40 Chaperone.Tgj1热休克蛋白40伴侣蛋白相互作用组分析
Proteomes. 2023 Mar 1;11(1):9. doi: 10.3390/proteomes11010009.
4
Glycosylation Analysis of Feline Small Intestine Following Infection.感染后猫小肠的糖基化分析
Animals (Basel). 2022 Oct 20;12(20):2858. doi: 10.3390/ani12202858.
5
The E3 Ubiquitin Ligase Adaptor Protein Skp1 Is Glycosylated by an Evolutionarily Conserved Pathway That Regulates Protist Growth and Development.E3泛素连接酶衔接蛋白Skp1通过一条进化上保守的途径进行糖基化修饰，该途径调控原生生物的生长和发育。
J Biol Chem. 2016 Feb 26;291(9):4268-80. doi: 10.1074/jbc.M115.703751. Epub 2015 Dec 30.
6
Sequence Variation in HSP40 Gene among 16 Toxoplasma gondii Isolates from Different Hosts and Geographical Locations.来自不同宿主和地理位置的16株刚地弓形虫分离株HSP40基因的序列变异
Biomed Res Int. 2015;2015:209792. doi: 10.1155/2015/209792. Epub 2015 Nov 3.
7
Toxoplasma gondii Hsp90: potential roles in essential cellular processes of the parasite.刚地弓形虫热休克蛋白90：在该寄生虫基本细胞过程中的潜在作用
Parasitology. 2014 Aug;141(9):1138-47. doi: 10.1017/S0031182014000055. Epub 2014 Feb 21.
8
DNA prime and peptide boost immunization protocol encoding the Toxoplasma gondii GRA4 induces strong protective immunity in BALB/c mice.编码刚地弓形虫GRA4的DNA初免和肽加强免疫方案可在BALB/c小鼠中诱导强烈的保护性免疫。
BMC Infect Dis. 2013 Oct 23;13:494. doi: 10.1186/1471-2334-13-494.
本文引用的文献
1
Toxoplasma gondii lysine acetyltransferase GCN5-A functions in the cellular response to alkaline stress and expression of cyst genes.刚地弓形虫赖氨酸乙酰转移酶 GCN5-A 在细胞响应碱性应激和囊虫基因表达中发挥作用。
PLoS Pathog. 2010 Dec 16;6(12):e1001232. doi: 10.1371/journal.ppat.1001232.
2
Intracellular protozoan parasites of humans: the role of molecular chaperones in development and pathogenesis.人类细胞内原生动物寄生虫：分子伴侣在发育和发病机制中的作用。
Protein Pept Lett. 2011 Feb;18(2):143-57. doi: 10.2174/092986611794475002.
3
The Hsp90 co-chaperone p23 of Toxoplasma gondii: Identification, functional analysis and dynamic interactome determination.刚地弓形虫的热休克蛋白90共伴侣蛋白p23：鉴定、功能分析及动态相互作用组测定
Mol Biochem Parasitol. 2010 Aug;172(2):129-40. doi: 10.1016/j.molbiopara.2010.04.004. Epub 2010 Apr 18.
4
I-TASSER: a unified platform for automated protein structure and function prediction.I-TASSER：一个用于自动化蛋白质结构和功能预测的统一平台。
Nat Protoc. 2010 Apr;5(4):725-38. doi: 10.1038/nprot.2010.5. Epub 2010 Mar 25.
5
Malaria heat shock proteins: drug targets that chaperone other drug targets.疟疾热休克蛋白：陪伴其他药物靶点的药物靶点。
Infect Disord Drug Targets. 2010 Jun;10(3):147-57. doi: 10.2174/187152610791163417.
6
I-TASSER: fully automated protein structure prediction in CASP8.I-TASSER：在 CASP8 中全自动的蛋白质结构预测。
Proteins. 2009;77 Suppl 9(Suppl 9):100-13. doi: 10.1002/prot.22588.
7
Improving physical realism, stereochemistry, and side-chain accuracy in homology modeling: Four approaches that performed well in CASP8.提高同源建模中的物理真实性、立体化学和侧链准确性：在 CASP8 中表现出色的四种方法。
Proteins. 2009;77 Suppl 9(Suppl 9):114-22. doi: 10.1002/prot.22570.
8
Toxoplasma H2A variants reveal novel insights into nucleosome composition and functions for this histone family.弓形虫H2A变体为该组蛋白家族的核小体组成和功能揭示了新的见解。
J Mol Biol. 2009 Sep 11;392(1):33-47. doi: 10.1016/j.jmb.2009.07.017. Epub 2009 Jul 14.
9
Protein wrapping: a molecular marker for association, aggregation and drug design.蛋白质包裹：关联、聚集及药物设计的分子标记
Chem Soc Rev. 2008 Nov;37(11):2373-82. doi: 10.1039/b804150b. Epub 2008 Sep 15.
10
Conserved central domains control the quaternary structure of type I and type II Hsp40 molecular chaperones.保守的中央结构域控制着I型和II型Hsp40分子伴侣的四级结构。
J Mol Biol. 2008 Oct 31;383(1):155-66. doi: 10.1016/j.jmb.2008.08.019. Epub 2008 Aug 14.
Zotero 插件