Ben-Ezra J, Sheibani K, Hwang D L, Lev-Ran A
James Irvine Center, City of Hope National Medical Center, Duarte, California 91010.
Am J Pathol. 1990 Oct;137(4):755-9.
Epidermal growth factor (EGF) is known to be present in the alpha granules of human platelets; however the source of this EGF, ie, whether it is taken up by the platelets from the circulation, or whether it is packaged into the platelets from the megakaryocyte during thrombopoiesis, is unknown. To determine whether EGF is taken up by platelets, platelets for EGF receptors were assayed and it was attempted to detect uptake of EGF by the platelets from culture medium. Platelets were found to lack EGF receptors, and no uptake of EGF from the culture medium was detected. To assess whether EGF is packaged into platelets from the megakaryocyte, megakaryocytes in frozen section bone marrow cores were stained for EGF protein by immunohistochemistry, and it was demonstrated that EGF is present in megakaryocytes. In addition, staining of megakaryocytes by in situ hybridization for EGF mRNA demonstrated its presence in these cells. Therefore it is concluded that the source of EGF in human platelets is the megakaryocyte and that this EGF is synthesized in the megakaryocyte rather than being taken up from its environment.
已知表皮生长因子（EGF）存在于人类血小板的α颗粒中；然而，这种EGF的来源，即它是血小板从循环中摄取的，还是在血小板生成过程中由巨核细胞包装到血小板中的，尚不清楚。为了确定血小板是否摄取EGF，对血小板的EGF受体进行了检测，并试图检测血小板从培养基中摄取EGF的情况。结果发现血小板缺乏EGF受体，且未检测到血小板从培养基中摄取EGF。为了评估EGF是否由巨核细胞包装到血小板中，通过免疫组织化学对冷冻切片骨髓芯中的巨核细胞进行EGF蛋白染色，结果表明EGF存在于巨核细胞中。此外，通过原位杂交对巨核细胞进行EGF mRNA染色，证明其在这些细胞中存在。因此得出结论，人类血小板中EGF的来源是巨核细胞，且这种EGF是在巨核细胞中合成的，而非从其周围环境摄取。
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