Centro de Estudios de Proteínas, Facultad de Biología, Universidad de La Habana, Calle 25 entre J e I, Vedado, Municipio Plaza, La Habana 10400, Cuba.
Biochimie. 2012 Apr;94(4):968-74. doi: 10.1016/j.biochi.2011.12.016. Epub 2011 Dec 24.
Antimicrobial peptides have been found in mollusks and other sea animals. In this report, a crude extract of the marine snail Cenchritis muricatus was evaluated against human pathogens responsible for multiple deleterious effects and diseases. A peptide of 1485.26 Da was purified by reversed-phase HPLC and functionally characterized. This trypsinized peptide was sequenced by MS/MS technology, and a sequence (SRSELIVHQR), named Cm-p1 was recovered, chemically synthesized and functionally characterized. This peptide demonstrated the capacity to prevent the development of yeasts and filamentous fungi. Otherwise, Cm-p1 displayed no toxic effects against mammalian cells. Molecular modeling analyses showed that this peptide possible forms a single hydrophilic α-helix and the probable cationic residue involved in antifungal activity action is proposed. The data reported here demonstrate the importance of sea animals peptide discovery for biotechnological tools development that could be useful in solving human health and agribusiness problems.
抗菌肽已在软体动物和其他海洋动物中被发现。在本报告中，评估了海洋蜗牛 Cenchritis muricatus 的粗提物对多种有害影响和疾病的人类病原体的作用。通过反相 HPLC 纯化了 1485.26 Da 的肽，并对其进行了功能表征。通过 MS/MS 技术对该胰蛋白酶化肽进行测序，回收了一个序列（SRSELIVHQR），命名为 Cm-p1，并对其进行了化学合成和功能表征。该肽具有预防酵母菌和丝状真菌生长的能力。此外，Cm-p1 对哺乳动物细胞没有毒性作用。分子建模分析表明，该肽可能形成单个亲水α-螺旋，并且提出了可能参与抗真菌活性的阳离子残基。这里报道的数据表明，海洋动物肽的发现对于生物技术工具的开发非常重要，这些工具可能有助于解决人类健康和农业综合企业的问题。
