Institute of Nutrition, Friedrich Schiller University of Jena, Jena, Germany.
Photochem Photobiol. 2012 Mar-Apr;88(2):371-80. doi: 10.1111/j.1751-1097.2011.01072.x. Epub 2012 Jan 23.
In many recent publications, supposed athermal effects of water-filtered infrared A (wIRA) irradiation are discussed. Those effects are mainly attributed to wavelengths in the range from 780 to 1440 nm, and should not result from warming of cellular water or any aqueous medium surrounding the irradiated sample caused by wIRA absorption. Athermal effects are considered to be induced directly by absorption of different wavelengths of the wIRA spectrum by cellular molecules or structures except water. To distinguish between thermal and athermal effects, irradiated samples have to be subjected to a very effective and precise temperature homeostasis. Any experimental effects can only be attributed to pure athermal effects, if the temperature of the irradiated samples is verifiably constant and does not result in hyperthermia. Here, data of temperature distribution in Petri dishes of different types filled with aqueous medium are presented which were estimated by model calculation for different setups of cooling. Additionally, the real temperature development was directly measured. Such a cooling unit enables long-term application of high wIRA irradiances and large doses without any detectable warming of the irradiated samples, in single cell layers. Using such a setup, thermal and athermal effects can be compared and in addition to that quantified.
在许多近期的出版物中，讨论了水过滤红外 A（wIRA）辐照的假定非热效应。这些效应主要归因于 780 到 1440nm 范围内的波长，而不应归因于 wIRA 吸收导致细胞水或辐照样品周围任何含水介质的升温。非热效应被认为是由除水以外的细胞分子或结构对 wIRA 光谱的不同波长的吸收直接诱导的。为了区分热效应和非热效应，辐照样品必须进行非常有效和精确的温度恒定性。如果辐照样品的温度可验证地保持恒定，并且不会导致过热，则任何实验效应只能归因于纯非热效应。在这里，介绍了通过模型计算针对不同冷却设置估计的不同类型的含有水介质的培养皿中的温度分布数据。此外，还直接测量了实际的温度发展。这种冷却单元能够在单细胞层中长期应用高 wIRA 辐照度和大剂量，而辐照样品不会有任何可检测到的升温。使用这种设置，可以比较和定量热效应和非热效应。
