沙雷氏 Actinobaculum 是否会导致儿童尿路感染？
Actinobaculum schaalii, a cause of urinary tract infections in children?
机构信息
Department of Paediatrics, Viborg Regional Hospital, Viborg, Denmark.
出版信息
Acta Paediatr. 2012 May;101(5):e232-4. doi: 10.1111/j.1651-2227.2011.02586.x. Epub 2012 Jan 23.
AIM
To inform that Actinobaculum schaalii can colonize the urine and cause urinary tract infection in children.
METHODS
Urine samples were examined by wet smear microscopy, incubated in 5% CO(2) for 1-2 days, and species-specific real-time polymerase chain reaction (PCR) for A. schaalii was performed.
RESULTS
In 5 of the 29 screened urines, A. schaalii was found only by real-time PCR in quantities equivalent to ≥ 10(4) -10(5) CFU/mL. In addition, A. schaalii was found in quantities equivalent to ≥ 10(6) CFU/mL by both culture and PCR in two children with a urinary tract infection and large numbers of leucocytes in the urine.
CONCLUSION
Actinobaculum schaalii is CO(2)-dependent. Therefore, if there are clinical symptoms and/or a negative culture despite the presence of leucocytes in the urine, Gram staining and incubation in 5% CO(2) or species-specific real-time PCR should be performed to identify A. schaalii.
目的
告知沙雷氏 Actinobaculum 可以定植于尿液并引起儿童尿路感染。
方法
通过湿涂片显微镜检查尿液样本，在 5%CO₂中孵育 1-2 天，并进行针对 A. schaalii 的种特异性实时聚合酶链反应（PCR）。
结果
在 29 份筛查的尿液中，仅通过实时 PCR 在数量上相当于≥10⁴-10⁵CFU/mL 发现了 A. schaalii。此外，在两名尿路感染且尿液中有大量白细胞的儿童中，通过培养和 PCR 均发现 A. schaalii 的数量相当于≥10⁶CFU/mL。
结论
沙雷氏 Actinobaculum 是依赖 CO₂的。因此，如果有临床症状和/或尽管尿液中有白细胞但培养结果为阴性，则应进行革兰氏染色和在 5%CO₂中孵育或进行种特异性实时 PCR，以鉴定 A. schaalii。
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