Department of Radiology, Huashan Hospital, Fudan University, Shanghai, China.
Acad Radiol. 2012 Feb;19(2):159-65. doi: 10.1016/j.acra.2011.09.017.
The aim of this study was to investigate the effect of perfusion computed tomography (PCT) with acetazolamide (ACZ) challenge and compare it to 10% carbon dioxide (CO(2)) challenge in rat C6 glioma.
PCT was performed on 32 rats, including 20 with orthotopically implanted C6 gliomas and 12 serving as controls. Ten rats with gliomas and six normal rats underwent PCT with ACZ challenge. The other 10 rats with gliomas and six normal rats underwent PCT with 10% CO(2) challenge. The raw data were processed using Philips computed tomographic brain perfusion software. Perfusion parameters before and after the challenge were recorded. Percentage changes due to ACZ administration and 10% CO(2) challenge were calculated. Pearson's correlation coefficients were used to investigate relationships between percentage changes in perfusion parameters and vascular endothelial growth factor and microvessel density.
In C6 gliomas, percentage change in cerebral blood flow was significantly different between ACZ (72.73%) and 10% CO(2) (28.47%) challenge (P < .01). Percentage change in cerebral blood volume was 37.85% with ACZ and 24.69% with 10% CO(2) challenge (P = .02). In controls, percentage change in cerebral blood flow was significantly different between ACZ (117.42%) and 10% CO(2) (65.86%) challenge (P < .01). For percentage change in cerebral blood volume, there was no significant difference between ACZ (107.51%) and 10% CO(2) (92.95%) challenge. Significant correlations were observed among percentage changes in vascular endothelial growth factor, microvessel density, and cerebral blood volume (P < .01). Percentage change in cerebral blood flow correlated well with vascular endothelial growth factor.
The results of this study indicate that PCT with ACZ challenge is a more reliable technique compared to 10% CO(2) challenge for the quantitative evaluation of microcirculation in gliomas.
本研究旨在探讨使用乙酰唑胺（ACZ）灌注 CT（PCT）的效果，并与 10%二氧化碳（CO2）在大鼠 C6 神经胶质瘤中的作用进行比较。
对 32 只大鼠进行了 PCT 检查，其中 20 只大鼠接受了原位植入的 C6 神经胶质瘤，12 只大鼠作为对照组。10 只胶质瘤大鼠和 6 只正常大鼠接受了 ACZ 挑战的 PCT，另外 10 只胶质瘤大鼠和 6 只正常大鼠接受了 10%CO2 挑战的 PCT。原始数据使用飞利浦 CT 脑灌注软件进行处理。记录挑战前后的灌注参数。计算因 ACZ 给药和 10%CO2 挑战引起的百分比变化。使用 Pearson 相关系数研究灌注参数的百分比变化与血管内皮生长因子和微血管密度之间的关系。
在 C6 神经胶质瘤中，ACZ（72.73%）和 10%CO2（28.47%）挑战之间的脑血流百分比变化差异有统计学意义（P <.01）。ACZ 时脑血容量的百分比变化为 37.85%，10%CO2 时为 24.69%（P =.02）。在对照组中，ACZ（117.42%）和 10%CO2（65.86%）挑战之间的脑血流百分比变化差异有统计学意义（P <.01）。ACZ（107.51%）和 10%CO2（92.95%）挑战之间脑血容量百分比变化无显著差异。血管内皮生长因子、微血管密度和脑血容量的百分比变化之间存在显著相关性（P <.01）。脑血流百分比变化与血管内皮生长因子密切相关。
本研究结果表明，与 10%CO2 挑战相比，ACZ 挑战的 PCT 是一种更可靠的技术，可用于定量评估胶质瘤中的微循环。
