Department of Urology, Center of Nephrology, The Second affiliated Hospital of the Third Military Medical University, Chongqing 400037, China.
Genes Cells. 2012 Jan;17(1):53-64. doi: 10.1111/j.1365-2443.2011.01572.x.
Ischemia and subsequent reperfusion (I/R) damage kidney tubular cells and consequently impair renal function. Rabbit bone marrow mesenchymal stem cells (BM-MSCs) expressing human bone morphogenic protein-7 (hBMP-7) regenerated tubular cells and improved renal function in a kidney I/R model. Rabbits were injected immediately after I/R with one of the following: (i) hBMP-7-transduced BM-MSCs (BM-MSCshBMP-7); (ii) enhanced green fluorescent protein-transduced BM-MSCs (BM-MSCsEGFP); or (iii) PBS. The activity of superoxide dismutase (SOD) was higher, and the amount of malondialdehyde (MDA) was lower in the BM-MSCshBMP-7 group than in the BM-MSCsEGFP group. Both the BM-MSCshBMP-7 group and the BM-MSCsEGFP group had higher SOD activity and lower amounts of MDA than the PBS group. Bcl-2- and Bcl-2-associated X protein levels, and other variables, indicated the regeneration of the kidney in both experimental groups. However, the BM-MSCs (hBMP-7) group showed higher activity than the BM-MSCsEGFP group, indicating that the combined strategy of BM-MSC transplantation with hBMP-7 gene therapy could be a useful approach for the treatment of renal IRI.
缺血和随后的再灌注（I/R）损伤肾小管细胞，从而损害肾功能。表达人骨形态发生蛋白-7（hBMP-7）的兔骨髓间充质干细胞（BM-MSCs）在肾 I/R 模型中再生管状细胞并改善肾功能。在 I/R 后立即向兔子注射以下一种物质：（i）转染 hBMP-7 的 BM-MSCs（BM-MSCshBMP-7）；（ii）转染增强型绿色荧光蛋白的 BM-MSCs（BM-MSCsEGFP）；或（iii）PBS。BM-MSCshBMP-7 组的超氧化物歧化酶（SOD）活性更高，丙二醛（MDA）含量更低，而 BM-MSCsEGFP 组的 SOD 活性更高，MDA 含量更低。与 PBS 组相比，BM-MSCshBMP-7 组和 BM-MSCsEGFP 组的 SOD 活性更高，MDA 含量更低。Bcl-2-和 Bcl-2 相关 X 蛋白水平以及其他变量表明两组肾脏均有再生。然而，BM-MSCs（hBMP-7）组的活性高于 BM-MSCsEGFP 组，表明 BM-MSC 移植与 hBMP-7 基因治疗的联合策略可能是治疗肾 IRI 的一种有效方法。
