佛罗里达州中北部地区野生猫科动物中流感病毒的监测。
Surveillance of feral cats for influenza A virus in north central Florida.
机构信息
Department of Infectious Diseases, University of Georgia, Athens, GA, USA.
出版信息
Influenza Other Respir Viruses. 2012 Sep;6(5):341-7. doi: 10.1111/j.1750-2659.2011.00325.x. Epub 2011 Dec 30.
BACKGROUND
Transmission of highly pathogenic avian influenza and the recent pandemic H1N1 viruses to domestic cats and other felids creates concern because of the morbidity and mortality associated with human infections as well as disease in the infected animals. Experimental infections have demonstrated transmission of influenza viruses in cats.
OBJECTIVES
An epidemiologic survey of feral cats was conducted to determine their exposure to influenza A virus.
METHODS
Feral cat sera and oropharyngeal and rectal swabs were collected from November 2008 through July 2010 in Alachua County, FL and were tested for evidence of influenza A virus infection by virus isolation, PCR, and serological assay.
RESULTS AND CONCLUSIONS
No virus was isolated from any of 927 cats examined using MDCK cell or embryonated chicken egg culture methods, nor was viral RNA detected by RT-PCR in 200 samples tested. However, 0.43% of cats tested antibody positive for influenza A by commercial ELISA. These results suggest feral cats in this region are at minimal risk for influenza A virus infection.
背景
高致病性禽流感病毒和最近的 H1N1 大流行性流感病毒向家猫和其他猫科动物的传播引起了人们的关注，因为人类感染以及感染动物的疾病会导致发病率和死亡率上升。实验感染已证明流感病毒在家猫之间的传播。
目的
对野猫进行了流行病学调查，以确定它们接触甲型流感病毒的情况。
方法
于 2008 年 11 月至 2010 年 7 月，在佛罗里达州阿拉楚阿县采集了野猫的血清和口咽及直肠拭子，并通过病毒分离、PCR 和血清学检测来检测流感 A 病毒感染的证据。
结果和结论
用 MDCK 细胞或鸡胚培养方法检查的 927 只猫中均未分离到病毒，200 个检测样本中也未通过 RT-PCR 检测到病毒 RNA。然而，通过商业 ELISA 检测，有 0.43%的猫对甲型流感病毒呈抗体阳性。这些结果表明，该地区的野猫感染甲型流感病毒的风险极小。
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