Piano M R, Ferguson J L, Melchior C L
Department of Physiology and Biophysics, College of Medicine, University of Illinois, Chicago 60680.
Alcohol Clin Exp Res. 1990 Aug;14(4):616-22. doi: 10.1111/j.1530-0277.1990.tb01212.x.
The purpose of these experiments was to determine whether or not tolerance develops to the effect of 3.0 g/kg ethanol on total and regional splanchnic blood flow in male Wistar rats. The animals were given the Lieber-DeCarli liquid diet containing ethanol for 10 days; ethanol-fed animals were withdrawn 24 hr prior to experiments. Regional blood flow and cardiac output (CO) were measured by the reference microsphere technique after an intraperitoneal injection of 3.0 g/kg of ethanol. Acute ethanol administration produced early nonsustained increases in portal vein blood flow in animals fed ethanol for 10 days and withdrawn for 24 hr and in control animals. However, after chronic exposure to ethanol, the pattern of increase in blood flow in response to ethanol in the splanchnic organs was different between the ethanol-fed and control groups. Increases in portal vein flow in control groups were due to concomitant increases in small intestinal, colonic, and cecal blood flow while the increase in the ethanol-fed group was due to a rise in small intestinal and stomach blood flow. The increase in stomach blood flow that occurred in the animals treated chronically with ethanol may be viewed as a conditioned response to ethanol, since this was not found in the control group. These results, demonstrate that the pattern of increase in blood flow in the splanchnic organs produced by an acute dose of ethanol depends on the animal's previous exposure to ethanol.
这些实验的目的是确定雄性Wistar大鼠对3.0 g/kg乙醇对总内脏血流量和局部内脏血流量的影响是否会产生耐受性。给动物喂食含乙醇的Lieber-DeCarli液体饮食10天；在实验前24小时停止给喂食乙醇的动物喂食。在腹腔注射3.0 g/kg乙醇后，通过参考微球技术测量局部血流量和心输出量（CO）。急性给予乙醇会使喂食乙醇10天并停喂24小时的动物以及对照动物的门静脉血流量早期出现非持续性增加。然而，长期接触乙醇后，乙醇喂养组和对照组内脏器官对乙醇反应的血流量增加模式有所不同。对照组门静脉血流量的增加是由于小肠、结肠和盲肠血流量同时增加，而乙醇喂养组的增加是由于小肠和胃血流量增加。长期用乙醇处理的动物胃血流量增加可被视为对乙醇的条件反应，因为对照组未发现这种情况。这些结果表明，急性剂量乙醇引起的内脏器官血流量增加模式取决于动物先前接触乙醇的情况。
