Institute of Biochemical Engineering, Technische Universität München, Boltzmannstrasse 15, Garching, Germany.
Biotechnol J. 2012 May;7(5):656-61. doi: 10.1002/biot.201100418. Epub 2012 Jan 18.
Acetone-butanol-ethanol (ABE) fermentation by Clostridium acetobutylicum has been extensively studied in recent years because the organism is recognized as an excellent butanol producer. A parallel bioreactor system with 48 stirred-tank bioreactors on a 12 mL scale was evaluated for batch cultivations of the strictly anaerobic, butanol-producing C. acetobutylicum ATCC 824. Continuous gassing with nitrogen gas was applied to control anaerobic conditions. Process performances of ABE batch fermentations on a milliliter scale were identical to the liter-scale stirred-tank reactor if reaction conditions were identical on the different scales (e.g., initial medium, pH, temperature, specific evaporation rates, specific power input by the stirrers). The effects of varying initial ammonia concentrations (0.1-4.4 g L(-1) ) were studied in parallel with respect to glucose consumption and butanol production of C. acetobutylicum ATCC 824 as a first application example. The highest butanol yield of 33% (mol mol(-1) ) was observed at initial ammonia concentrations of 0.5 and 1.1 g L(-1) . This is the first report on the successful application of a 48 parallel stirred-tank bioreactor system for reaction engineering studies of strictly anaerobic microorganisms at the milliliter scale.
近年来，由于该生物被认为是一种优秀的丁醇生产菌，丙酮丁醇乙醇（ABE）发酵已被广泛研究。一个具有 48 个搅拌罐生物反应器的平行生物反应器系统，在 12 毫升规模上，用于分批培养严格厌氧的丁醇生产菌丙酮丁醇梭菌 ATCC 824。连续通氮气用于控制厌氧条件。如果在不同规模上的反应条件相同（例如初始培养基、pH 值、温度、特定蒸发率、搅拌器的特定功率输入），那么在毫升规模上的 ABE 分批发酵的过程性能与升规模搅拌罐反应器相同。作为第一个应用实例，研究了初始氨浓度（0.1-4.4 g L（-1））的变化对丙酮丁醇梭菌 ATCC 824 的葡萄糖消耗和丁醇生产的影响。在初始氨浓度为 0.5 和 1.1 g L（-1）时，观察到最高的丁醇产率为 33%（摩尔摩尔（-1））。这是首次成功应用 48 个平行搅拌罐生物反应器系统，在毫升规模上对严格厌氧微生物进行反应工程研究的报告。
