Department of Chemical and Materials Engineering, Tunghai University, Taichung, Taiwan, ROC.
Bioresour Technol. 2013 Oct;145:224-8. doi: 10.1016/j.biortech.2012.11.039. Epub 2012 Nov 17.
High butanol accumulation is due to feedback inhibition which leads to the low butanol productivity observed in acetone-butanol-ethanol (ABE) fermentation. The aim of this study is to use biodiesel as an extractant for the in situ removal of butanol from the broth. The results indicate that adding biodiesel as an extractant at the beginning of fermentation significantly enhances butanol production. No significant toxicity of biodiesel on the growth of Clostridium acetobutylicum is observed. In the fed-batch operation with glucose feeding, the maximum total butanol obtained is 31.44 g/L, as compared to the control batch (without the addition of biodiesel) at 9.85 g/L. Moreover, the productivity obtained is 0.295 g/L h in the fed-batch, which is higher than that of 0.185 g/L h for the control batch. The in situ butanol removal by the addition of biodiesel has great potential for commercial ABE production.
高丁醇积累是由于反馈抑制导致在丙酮丁醇乙醇（ABE）发酵中观察到的低丁醇生产力。本研究的目的是使用生物柴油作为从发酵液中就地提取丁醇的萃取剂。结果表明，在发酵开始时添加生物柴油作为萃取剂可显著提高丁醇的产量。生物柴油对丙酮丁醇梭菌的生长没有明显的毒性。在葡萄糖进料的分批补料操作中，与对照批次（未添加生物柴油）的 9.85 g/L 相比，获得的最大总丁醇量为 31.44 g/L。此外，补料批次的产率为 0.295 g/L·h，高于对照批次的 0.185 g/L·h。通过添加生物柴油从发酵液中就地去除丁醇，在商业 ABE 生产中具有很大的潜力。
