Tannich E, Tümmler M, Arnold H H, Lingelbach K
Bernhard Nocht-Institute of Tropical Medicine, Hamburg, Federal Republic of Germany.
Anal Biochem. 1990 Aug 1;188(2):255-8. doi: 10.1016/0003-2697(90)90602-6.
A simple procedure is described for the efficient deletion of large DNA sequences. The method involves a combination of oligonucleotide-directed mutagenesis in bacteriophage M13 and amplification of the mutagenized product by polymerase chain reaction. In contrast to other protocols employing polymerase chain reaction, synthesis of only one specific primer is required. The efficiency of heteroduplex formation between mutagenic primers directing large deletions and single-stranded template is discussed.
本文描述了一种用于高效删除大片段DNA序列的简单方法。该方法包括噬菌体M13中寡核苷酸定向诱变与通过聚合酶链反应扩增诱变产物的结合。与其他使用聚合酶链反应的方案不同，该方法仅需要合成一种特异性引物。文中还讨论了引导大片段缺失的诱变引物与单链模板之间异源双链体形成的效率。
