Group of Vascular Regeneration Research, Institute of Biomedical Research and Innovation, RIKEN Center for Developmental Biology, Kobe, Japan.
PLoS One. 2011;6(12):e28639. doi: 10.1371/journal.pone.0028639. Epub 2011 Dec 28.
Numerous endothelial progenitor cell (EPC)-related investigations have been performed in mouse experiments. However, defined characteristics of mouse cultured EPC have not been examined. We focused on fast versus slow adherent cell population in bone marrow mononuclear cells (BMMNCs) in culture and examined their characteristics. After 24 h-culture of BMMNCs, attached (AT) cells and floating (FL) cells were further cultured in endothelial differentiation medium separately. Immunological and molecular analyses exhibited more endothelial-like and less monocyte/macrophage-like characteristics in FL cells compared with AT cells. FL cells formed thick/stable tube and hypoxia or shear stress overload further enhanced these endothelial-like features with increased angiogenic cytokine/growth factor mRNA expressions. Finally, FL cells exhibited therapeutic potential in a mouse myocardial infarction model showing the specific local recruitment to ischemic border zone and tissue preservation. These findings suggest that slow adherent (FL) but not fast attached (AT) BMMNCs in culture are EPC-rich population in mouse.
大量与内皮祖细胞（EPC）相关的研究已经在小鼠实验中进行。然而，尚未对培养的小鼠 EPC 的特征进行定义。我们专注于骨髓单个核细胞（BMMNC）培养中快速和缓慢黏附细胞群体，并对其特征进行了研究。BMMNC 培养 24 小时后，将附着（AT）细胞和漂浮（FL）细胞分别在内皮细胞分化培养基中进一步培养。免疫和分子分析显示，与 AT 细胞相比，FL 细胞具有更多的内皮样特征和更少的单核细胞/巨噬细胞样特征。FL 细胞形成厚而稳定的管状结构，缺氧或剪应力过载进一步增强了这些内皮样特征，同时增加了血管生成细胞因子/生长因子 mRNA 的表达。最后，FL 细胞在小鼠心肌梗死模型中表现出治疗潜力，显示出对缺血边界区的特异性局部募集和组织保护。这些发现表明，培养中的缓慢黏附（FL）而非快速黏附（AT）BMMNC 是富含 EPC 的细胞群体。
