Zeng Wen, Lei Qiaoling, Ma Jiao, Ju Rong
Department of Neonatology, Chengdu Women's and Children's Central Hospital, School of Medicine, University of Electronic Science and Technology of China, Chengdu, Sichuan 611731, P.R. China.
Exp Ther Med. 2020 Mar;19(3):2171-2178. doi: 10.3892/etm.2020.8468. Epub 2020 Jan 23.
Endothelial progenitor cells (EPCs) have protective roles in ischemic injury due to their ability to improve endothelial function and modulate angiogenesis. Microvesicles (MVs) are small membrane particles released by various cell types, including EPCs, which affect various target cells by transferring carried genetic information, including microRNAs (miRNAs/miRs). Depending on the stimuli and cell types, MVs exert different functions. In the present study, oxygen-glucose deprivation (OGD) was used to mimic ischemic-hypoxic (HI) insult, where the effects of HI insult on EPC-derived MVs (EPC-MVs) were subsequently investigated. OGD induced Ca influx in EPCs and increased the release of EPC-MVs compared with normoxic conditions. In addition, MVs prepared from EPCs cultured under normoxic conditions or OGD conditions (OGD-EMVs) had the ability to stimulate the proliferation of EPCs. Furthermore, OGD-EMVs induced stronger effects on proliferation, which may be associated with the upregulation of miR-210 in EPC-MVs. In conclusion, the present results indicated that HI insult promoted the release of MVs from EPCs and upregulated miR-210 in MVs, leading to positive modulation of the proliferation of EPCs cultured under normoxic conditions.
内皮祖细胞（EPCs）因其改善内皮功能和调节血管生成的能力，在缺血性损伤中具有保护作用。微泡（MVs）是包括EPCs在内的多种细胞类型释放的小膜颗粒，其通过转移携带的遗传信息（包括微小RNA（miRNAs/miRs））来影响各种靶细胞。根据刺激因素和细胞类型的不同，微泡发挥不同的功能。在本研究中，采用氧糖剥夺（OGD）来模拟缺血缺氧（HI）损伤，随后研究HI损伤对EPC来源的微泡（EPC-MVs）的影响。与常氧条件相比，OGD诱导EPCs中的钙内流并增加EPC-MVs的释放。此外，从常氧条件或OGD条件下培养的EPCs制备的微泡（OGD-EMVs）具有刺激EPCs增殖的能力。此外，OGD-EMVs对增殖的诱导作用更强，这可能与EPC-MVs中miR-210的上调有关。总之，本研究结果表明，HI损伤促进了EPCs释放微泡并上调了微泡中的miR-210，从而对常氧条件下培养的EPCs的增殖产生正向调节作用。
