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评估三种市售甲型流感病毒特异性阻断酶联免疫吸附测定法用于猪甲型流感病毒感染血清流行病学研究。

Evaluation of three commercially available influenza A type-specific blocking enzyme-linked immunosorbent assays for seroepidemiological studies of influenza A virus infection in pigs.

作者信息

Tse Maying, Kim Mia, Chan Chung-Hei, Ho Po-Lai, Ma Siu-Kit, Guan Yi, Peiris J S M

机构信息

State Key Laboratory of Emerging Infectious Diseases & School of Public Health, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong Special Administrative Region, China.

出版信息

Clin Vaccine Immunol. 2012 Mar;19(3):334-7. doi: 10.1128/CVI.05358-11. Epub 2012 Jan 4.

Abstract

The reverse zoonotic transmission of the pandemic H1N1 2009 influenza virus to swine necessitates enhanced surveillance of swine for influenza virus infection. Using a well-characterized panel of naturally infected swine sera, we evaluated and optimized the performances of three commercially available competitive enzyme-linked immunosorbent assays (ELISAs), namely, the IDEXX Influenza A Ab test, IDEXX AI MultiS-Screen Ab test, and IDVet ID Screen influenza A antibody competition ELISA, for detecting influenza A virus-reactive antibodies in swine. Receiver operating characteristic (ROC) analysis suggests that adjustment of the manufacturer-recommended cutoff values optimizes the sensitivity and specificity of these assays, making them applicable for seroepidemiology studies of swine influenza. Using such optimized cutoff levels, the sensitivity and specificity of the IDEXX Influenza A Ab test were 86% and 89%, respectively; those for the IDEXX AI MultiS-Screen Ab test were 91% and 87%, respectively; and those for the IDVet ID Screen influenza A test were 95% and 79%, respectively.

摘要

2009年甲型H1N1流感病毒向猪的反向人畜共患病传播,使得加强对猪流感病毒感染的监测成为必要。我们使用一组特征明确的自然感染猪血清,评估并优化了三种市售竞争性酶联免疫吸附测定(ELISA),即IDEXX甲型流感抗体检测、IDEXX禽流感多重筛选抗体检测和IDVet ID Screen甲型流感抗体竞争ELISA,用于检测猪体内甲型流感病毒反应性抗体。受试者操作特征(ROC)分析表明,调整制造商推荐的临界值可优化这些检测方法的敏感性和特异性,使其适用于猪流感的血清流行病学研究。使用这些优化的临界水平时,IDEXX甲型流感抗体检测的敏感性和特异性分别为86%和89%;IDEXX禽流感多重筛选抗体检测的敏感性和特异性分别为91%和87%;IDVet ID Screen甲型流感检测的敏感性和特异性分别为95%和79%。

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