血清中分泌型去唾液酸糖蛋白受体 sH2a 的水平持续下降，与肝硬化有关。
Constant serum levels of secreted asialoglycoprotein receptor sH2a and decrease with cirrhosis.
机构信息
Department of Cell Research and Immunology, George Wise Faculty of Life Sciences, Tel Aviv University, Tel Aviv 69978, Israel.
出版信息
World J Gastroenterol. 2011 Dec 28;17(48):5305-9. doi: 10.3748/wjg.v17.i48.5305.
Abstract
AIM
To investigate the existence and levels of sH2a, a soluble secreted form of the asialoglycoprotein receptor in human serum.
METHODS
Production of recombinant sH2a and development of a monoclonal antibody and an enzyme-linked immunosorbent assay (ELISA). This assay was used to determine the presence and concentration of sH2a in human sera of individuals of both sexes and a wide range of ages.
RESULTS
The recombinant protein was produced successfully and a specific ELISA assay was developed. The levels of sH2a in sera from 62 healthy individuals varied minimally (147 ± 19 ng/mL). In contrast, 5 hepatitis C patients with cirrhosis showed much decreased sH2a levels (50 ± 9 ng/mL).
CONCLUSION
Constant sH2a levels suggest constitutive secretion from hepatocytes in healthy individuals. This constant level and the decrease with cirrhosis suggest a diagnostic potential.
摘要
目的
研究人血清中作为去唾液酸糖蛋白受体可溶性分泌形式的 sH2a 是否存在及其水平。
方法
生产重组 sH2a 并开发单克隆抗体和酶联免疫吸附测定（ELISA）。该测定法用于确定两性和广泛年龄段个体的人血清中 sH2a 的存在和浓度。
结果
成功生产重组蛋白，并开发了一种特异性 ELISA 测定法。来自 62 名健康个体的血清中 sH2a 的水平变化很小（147 ± 19 ng/mL）。相比之下，5 名患有肝硬化的丙型肝炎患者的 sH2a 水平明显降低（50 ± 9 ng/mL）。
结论
健康个体中 sH2a 水平的恒定表明其从肝细胞持续分泌。这种恒定水平和肝硬化时的降低表明其具有诊断潜力。
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