Institute of Marine and Environmental Technology, University of Maryland Center for Environmental Science, Baltimore, MD 21202, USA.
J Fish Biol. 2012 Jan;80(1):110-30. doi: 10.1111/j.1095-8649.2011.03158.x. Epub 2011 Dec 5.
The entire mitochondrial genome of the striped bass Morone saxatilis was sequenced together with the mitochondrial (mt) control regions of the white bass Morone chrysops, white perch Morone americana, yellow bass Morone mississippiensis, spotted seabass Dicentrarchus punctatus, European seabass Dicentrarchus labrax and the Japanese seabass Lateolabrax japonicus. The resultant 17 580 base pair circular genome of M. saxatilis contains 38 genes (13 proteins, 23 transfer RNAs and two ribosomal RNAs) and a control region bordered by the proline and phenylalanine mitochondrial tRNAs. Gene arrangement was similar to other vertebrates, except that the mt-nd6 gene was found within the control region rather than the canonical position between the mt-nd5 and mt-cyb genes. This translocation was found in all the Morone and Dicentrarchus species studied without functional copies or pseudogenes in the ancestral position. In L. japonicus, the mt-nd6 gene was found in the canonical position without evidence of an mt-nd6 gene in the control region. A Bayesian analysis of these and published mt-nd6 sequences from 45 other Perciformes grouped the Morone and Dicentrarchus species monophyletically with a probability of 1·00 with respect to L. japonicus and all other perciforms, and placed the Dicentrarchus species in the basal position. These data reinforce current placement of L. japonicus outside the Moronidae and provide a clear evolutionary character to define this family. The phylogeny of the Moronidae presented here also supports the hypothesis of an anadromous common ancestor to this family that gave rise to the North American estuarine and freshwater species. A series of tandem repeats previously reported in M. saxatilis was found in the control region of all Morone species between the mt-nd6 and mt-rnr1 genes, but not in either Dicentrarchus species, which reinforces the continued use of these two separate genera.
石斑鱼 Morone saxatilis 的完整线粒体基因组与白鲈 Morone chrysops、美洲白鲈 Morone americana、黄鲈 Morone mississippiensis、斑点石斑鱼 Dicentrarchus punctatus、欧洲鲈鱼 Dicentrarchus labrax 和日本鲈鱼 Lateolabrax japonicus 的线粒体（mt）控制区一起进行了测序。M. saxatilis 的 17580 个碱基对圆形基因组包含 38 个基因（13 个蛋白质、23 个转移 RNA 和 2 个核糖体 RNA）和一个由脯氨酸和苯丙氨酸线粒体 tRNA 边界的控制区。基因排列与其他脊椎动物相似，除了 mt-nd6 基因位于控制区而不是 mt-nd5 和 mt-cyb 基因之间的典型位置之外。这种易位在所有研究的 Morone 和 Dicentrarchus 物种中都发现了，在前体位置没有功能性副本或假基因。在 L. japonicus 中，mt-nd6 基因位于典型位置，而在控制区没有发现 mt-nd6 基因的证据。对这些基因和来自 45 种其他鲈形目动物的已发表 mt-nd6 序列的贝叶斯分析将 Morone 和 Dicentrarchus 物种与 L. japonicus 和所有其他鲈形目动物聚为一组，具有 1.00 的概率，而 Dicentrarchus 物种位于基部。这些数据加强了当前将 L. japonicus 置于 Moronidae 之外的位置，并提供了一个明确的进化特征来定义这个家族。这里提出的 Moronidae 系统发育也支持了这个家族的溯河洄游共同祖先的假说，这个假说导致了北美的河口和淡水物种。先前在 M. saxatilis 中报道的一系列串联重复序列在所有 Morone 物种的控制区中位于 mt-nd6 和 mt-rnr1 基因之间，但在两个 Dicentrarchus 物种中都没有，这加强了对这两个单独属的持续使用。
