Laboratory for Molecular Genetics and Animal Biotechnology, Faculty of Animal Sciences and Fisheries, Sari Agricultural Sciences and Natural Resources University, Sari, Iran.
Department of Basic Sciences, Sari Agricultural Sciences and Natural Resources University, Sari, Iran.
BMC Ecol Evol. 2023 Sep 26;23(1):55. doi: 10.1186/s12862-023-02166-2.
The sturgeon group has been economically significant worldwide due to caviar production. Sturgeons consist of 27 species in the world. Mitogenome data could be used to infer genetic diversity and investigate the evolutionary history of sturgeons. A limited number of complete mitogenomes in this family were sequenced. Here, we annotated the mitochondrial Huso huso genome, which revealed new aspects of this species.
In this species, the mitochondrial genome consisted of 13 genes encoding proteins, 22tRNA and 2rRNA, and two non-coding regions that followed other vertebrates. In addition, H. huso had a pseudo-tRNA-Glu between ND6 and Cytb and a 52-nucleotide tandem repeat with two replications in 12S rRNA. This duplication event is probably related to the slipped strand during replication, which could remain in the strand due to mispairing during replication. Furthermore, an 82 bp repeat sequence with three replications was observed in the D-loop control region, which is usually visible in different species. Regulatory elements were also seen in the control region of the mitochondrial genome, which included termination sequences and conserved regulatory blocks. Genomic compounds showed the highest conservation in rRNA and tRNA, while protein-encoded genes and nonencoded regions had the highest divergence. The mitochondrial genome was phylogenetically assayed using 12 protein-encoding genes.
In H. huso sequencing, we identified a distinct genome organization relative to other species that have never been reported. In recent years, along with the advancement in sequencing identified more genome rearrangements. However, it is an essential aspect of researching the evolution of the mitochondrial genome that needs to be recognized.
由于鱼子酱的生产，鲟鱼在世界范围内具有重要的经济意义。鲟鱼包括世界上的 27 个物种。线粒体基因组数据可用于推断遗传多样性并研究鲟鱼的进化历史。该家族中只有少数完整的线粒体基因组被测序。在这里，我们注释了西伯利亚鲟的线粒体基因组，揭示了该物种的新方面。
在该物种中，线粒体基因组由编码蛋白质的 13 个基因、22 个 tRNA 和 2 个 rRNA 以及遵循其他脊椎动物的两个非编码区组成。此外，H. huso 在 ND6 和 Cytb 之间具有假 tRNA-Glu 和在 12S rRNA 中有两个重复的 52 个核苷酸串联重复。这种重复事件可能与复制过程中的滑动链有关，由于复制过程中的错配，该链可能会保留下来。此外，在 D 环控制区观察到一个具有三个重复的 82 bp 重复序列，这在不同物种中通常可见。线粒体基因组的控制区也观察到了调节元件，包括终止序列和保守调节块。基因组化合物在 rRNA 和 tRNA 中表现出最高的保守性，而蛋白质编码基因和非编码区表现出最高的差异。使用 12 个蛋白质编码基因对线粒体基因组进行了系统发育分析。
在 H. huso 测序中，我们确定了与其他从未报道过的物种相对的独特基因组组织。近年来，随着测序技术的进步，发现了更多的基因组重排。然而，这是研究线粒体基因组进化所必需的一个重要方面。
