Institute of Environmental Science & Research Ltd, Christchurch Science Centre, P.O. Box 29-181, Christchurch 8540, New Zealand.
Ground Water. 2012 Sep-Oct;50(5):690-703. doi: 10.1111/j.1745-6584.2011.00904.x. Epub 2012 Jan 3.
This paper describes the in situ response of groundwater biofilms in an alluvial gravel aquifer system on the Canterbury Plains, New Zealand. Biofilms were developed on aquifer gravel, encased in fine mesh bags and suspended in protective columns in monitoring wells for at least 20 weeks. Four sites were selected in the same groundwater system where previous analyses indicated a gradient of increasing nitrate down the hydraulic gradient from Sites 1 to 4. Measurements during the current study classified the groundwater as oligotrophic. Biofilm responses to the nutrient gradients were assessed using bioassays, with biomass determined using protein and cellular and nucleic acid staining and biofilm activity using enzyme assays for lipid, carbohydrate, phosphate metabolism, and cell viability. In general, biofilm activity decreased as nitrate levels increased from Sites 1 to 4, with the opposite relationship for carbon and phosphorus concentrations. These results showed that the groundwater system supported biofilm growth and that the upper catchment supported efficient and productive biofilms (high ratio of activity per unit biomass).
本文描述了新西兰坎特伯雷平原冲积砾石含水层系统中地下水生物膜的原位响应。生物膜在含水层砾石上发育，用细网袋包裹，并悬挂在监测井中的保护柱中，至少 20 周。在同一地下水系统中选择了四个地点，此前的分析表明，从地点 1 到 4，硝酸盐沿水力梯度呈递增梯度。本研究期间的测量将地下水分类为贫营养型。使用生物测定法评估生物膜对养分梯度的响应，使用蛋白质和细胞及核酸染色法测定生物量，使用脂质、碳水化合物、磷酸盐代谢和细胞活力的酶测定法测定生物膜活性。一般来说，随着硝酸盐水平从地点 1 到 4 的增加，生物膜活性降低，而碳和磷浓度则相反。这些结果表明，地下水系统支持生物膜生长，并且上游集水区支持高效和多产的生物膜（每单位生物量的活性比高）。
