State Key Laboratory of Urban and Regional Ecology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, China.
FEMS Microbiol Ecol. 2012 Apr;80(1):146-58. doi: 10.1111/j.1574-6941.2011.01280.x. Epub 2012 Jan 9.
To understand the distribution and diversity of archaea in Chinese soils, the archaeal communities in a series of topsoils and soil profiles were investigated using quantitative PCR, T-RFLP combining sequencing methods. Archaeal 16S rRNA gene copy numbers, ranging from 4.96 × 10(6) to 1.30 × 10(8) copies g(-1) dry soil, were positively correlated with soil pH, organic carbon and total nitrogen in the topsoils. In the soil profiles, archaeal abundance was positively correlated with soil pH but negatively with depth profile. The relative abundance of archaea in the prokaryotes (sum of bacteria and archaea) ranged from 0.20% to 9.26% and tended to increase along the depth profile. T-RFLP and phylogenetic analyses revealed that the structure of archaeal communities in cinnamon soils, brown soils, and fluvo-aquic soils was similar and dominated by Crenarchaeota group 1.1b and 1.1a. These were different from those in red soils, which were dominated by Crenarchaeota group 1.3 and 1.1c. Canonical correspondence analysis indicated that the archaeal community was primarily influenced by soil pH.
为了了解中国土壤中古菌的分布和多样性，本研究采用定量 PCR、T-RFLP 结合测序方法，对一系列表层土和土壤剖面中的古菌群落进行了调查。古菌 16S rRNA 基因拷贝数范围为 4.96×10(6)至 1.30×10(8)拷贝 g(-1)干土，与表层土中的土壤 pH、有机碳和总氮呈正相关。在土壤剖面上，古菌丰度与土壤 pH 呈正相关，但与深度呈负相关。古菌在原核生物（细菌和古菌之和）中的相对丰度范围为 0.20%至 9.26%，并倾向于随深度剖面而增加。T-RFLP 和系统发育分析表明，肉桂土、棕壤和潮土中古菌群落的结构相似，主要由 Crenarchaeota 组 1.1b 和 1.1a 组成。而红壤中的古菌群落则主要由 Crenarchaeota 组 1.3 和 1.1c 组成。典范对应分析表明，古菌群落主要受土壤 pH 的影响。
