佐剂对病毒样颗粒诱导的 IgG 亚类的影响。

Effects of adjuvants on IgG subclasses elicited by virus-like particles.

机构信息

Lab, of Molecular Biology and Viral Oncogenesis, Istituto Nazionale Tumori Fond, G, Pascale, Naples-Italy.

出版信息

J Transl Med. 2012 Jan 5;10:4. doi: 10.1186/1479-5876-10-4.

BACKGROUND

Virus-Like Particles (VLPs) represent an efficient strategy to present and deliver conformational antigens to the immune system, inducing both arms of the adaptive immune response. Moreover, their particulate structure surrounded by cell membrane provides an adjuvanted effect to VLP-based immunizations. In the present study, the elicitation of different patterns of IgG subclasses by VLPs, administered in CpG ODN1826 or poly(I:C) adjuvants, has been evaluated in an animal model.

RESULTS

Adjuvanted VLPs elicited a higher titer of total specific IgG compared to VLPs alone. Furthermore, while VLPs alone induced a balanced TH2 pattern, VLPs formulated with either adjuvant elicited a TH1-biased IgG subclasses (IgG2a and IgG3), with poly(I:C) more potent than CpG ODN1826.

CONCLUSIONS

The results confirmed that adjuvants efficiently improve antigen immunogenicity and represent a suitable strategy to skew the adaptive immune response toward the differentiation of the desired T helper subset, also using VLPs as antigen.

背景

病毒样颗粒 (VLPs) 代表了一种将构象抗原递呈给免疫系统的有效策略，能够诱导适应性免疫反应的两个分支。此外，它们由细胞膜包围的颗粒状结构为基于 VLP 的免疫接种提供了佐剂效应。在本研究中，评估了在动物模型中，CpG ODN1826 或聚肌苷酸：聚胞苷酸（poly(I:C)）佐剂给药的 VLPs 引发不同 IgG 亚类模式的能力。

结果

与单独的 VLPs 相比，佐剂增强的 VLPs 引起了更高滴度的特异性总 IgG。此外，虽然单独的 VLPs 诱导出平衡的 TH2 模式，但用任何一种佐剂配制的 VLPs 均诱导出偏向 TH1 的 IgG 亚类（IgG2a 和 IgG3），其中 poly(I:C)比 CpG ODN1826 更有效。