采用 UHPLC/UV/MS 对育亨宾树皮及其相关膳食补充剂进行色谱指纹分析。

Chromatographic fingerprint analysis of yohimbe bark and related dietary supplements using UHPLC/UV/MS.

机构信息

U.S. Department of Agriculture, Agricultural Research Service, Beltsville Human Nutrition Research Center, Food Composition and Methods Development Laboratory, Beltsville, MD, USA.

出版信息

J Pharm Biomed Anal. 2012 Mar 5;61:142-9. doi: 10.1016/j.jpba.2011.11.013. Epub 2011 Nov 20.

DOI:10.1016/j.jpba.2011.11.013

PMID:22221902

Abstract

A practical ultra high-performance liquid chromatography (UHPLC) method was developed for fingerprint analysis of and determination of yohimbine in yohimbe barks and related dietary supplements. Good separation was achieved using a Waters Acquity BEH C(18) column with gradient elution using 0.1% (v/v) aqueous ammonium hydroxide and 0.1% ammonium hydroxide in methanol as the mobile phases. The study is the first reported chromatographic method that separates corynanthine from yohimbine in yohimbe bark extract. The chromatographic fingerprint analysis was applied to the analysis of 18 yohimbe commercial dietary supplement samples. Quantitation of yohimbine, the traditional method for analysis of yohimbe barks, were also performed to evaluate the results of the fingerprint analysis. Wide variability was observed in fingerprints and yohimbine content among yohimbe dietary supplement samples. For most of the dietary supplements, the yohimbine content was not consistent with the label claims.

摘要

建立了一种实用的超高效液相色谱（UHPLC）方法，用于分析育亨宾树皮及其相关膳食补充剂中的育亨宾的指纹图谱和含量。采用 Waters Acquity BEH C（18）柱，以 0.1%（v/v）氨水溶液和甲醇中的 0.1%氨水溶液作为流动相进行梯度洗脱，实现了良好的分离。该研究是首次报道的在育亨宾树皮提取物中分离育亨宾碱和科里纳宁的色谱方法。色谱指纹图谱分析应用于 18 种育亨宾商业膳食补充剂样品的分析。还进行了育亨宾碱的定量分析，这是分析育亨宾树皮的传统方法，以评估指纹图谱分析的结果。在育亨宾膳食补充剂样品中，指纹图谱和育亨宾含量存在广泛的变异性。对于大多数膳食补充剂，育亨宾碱的含量与标签声称不一致。