Industrial Program, Brazilian Bioethanol Science and Technology Laboratory - CTBE, Rua Giuseppe Maximo Scolfaro 10000, Pólo II de Alta Tecnologia, Caixa Postal 6192, CEP 13083-970 Campinas, São Paulo, Brazil.
Bioresour Technol. 2012 Mar;107:517-21. doi: 10.1016/j.biortech.2011.12.048. Epub 2011 Dec 17.
The on-site production of cellulases is an important strategy for the development of sustainable second-generation ethanol production processes. This study concerns the use of a specific cellulolytic enzyme complex for hydrolysis of pretreated sugar cane bagasse. Glycosyl hydrolases (FPase, xylanase, and β-glucosidase) were produced using a new strain of Trichoderma harzianum, isolated from the Amazon rainforest and cultivated under different conditions. The influence of the carbon source was first investigated using shake-flask cultures. Selected carbon sources were then further studied under different pH conditions using a stirred tank bioreactor. Enzymatic activities up to 121 FPU/g, 8000 IU/g, and 1730 IU/g of delignified steam-exploded bagasse+sucrose were achieved for cellulase, xylanase and β-glucosidase, respectively. This enzymatic complex was used to hydrolyze pretreated sugar cane bagasse. A comparative evaluation, using an enzymatic extract from Trichoderma reesei RUTC30, indicated similar performance of the T. harzianum enzyme complex, being a potential candidate for on-site production of enzymes.
现场生产纤维素酶是开发可持续第二代乙醇生产工艺的重要策略。本研究涉及使用特定的纤维素酶复合体系来水解预处理过的甘蔗渣。使用从亚马逊雨林中分离出的新型木霉（Trichoderma harzianum）菌株，在不同条件下培养生产糖苷水解酶（FPase、木聚糖酶和β-葡萄糖苷酶）。首先在摇瓶培养中研究了碳源的影响，然后在搅拌罐生物反应器中进一步研究了选定的碳源在不同 pH 条件下的影响。对于脱木质素的蒸汽爆破甘蔗渣+蔗糖，纤维素酶、木聚糖酶和β-葡萄糖苷酶的酶活分别达到了 121 FPU/g、8000 IU/g 和 1730 IU/g。该酶复合体系用于水解预处理过的甘蔗渣。使用里氏木霉（Trichoderma reesei）RUTC30 的酶提取物进行比较评估，表明木霉（Trichoderma harzianum）酶复合体系的性能相似，是现场生产酶的潜在候选者。
