School of Life Sciences and Chemical Technology, Ngee Ann Polytechnic, 535 Clementi Road, 599489, Singapore.
J Biosci Bioeng. 2020 Feb;129(2):242-249. doi: 10.1016/j.jbiosc.2019.08.016. Epub 2019 Sep 24.
Trichoderma harzianum EU2-77 was a mutant strain of the wild-type strain T. harzianum NP13a isolated in Singapore. A multi-mutagenesis one-screening (MMOS) method was developed to further improve strain EU2-77 and a new mutant EUA20 was obtained. It exhibited filter paper cellulase (FPase) activity up to 14.79 IU/mL within 6 days shake flask cultivation. Activities of FPase, endoglucanase, β-glucosidase, and xylanase, and protein content by EUA20 were respectively increased to 5.73, 4.35, 7.34, 1.80 and 2.70 folds. Using pretreated oil palm empty fruit bunch (OPEFB) and corncob powder as the substrates, strain EUA20 presented approximate 6.52 and 8.80 IU/ml FPase activity. Reducing sugar yield of 615.8 and 636.8 mg/g biomass were respectively obtained for OPEFB and corncob powder using cellulolytic enzymes of strain EUA20. Our results demonstrated that mutant strain EUA20 had great potential in on-site cellulase production for effective biomass bioconversion.
哈茨木霉 EU2-77 是从新加坡分离的野生型哈茨木霉 NP13a 的突变株。建立了一种多诱变一筛选（MMOS）方法来进一步提高菌株 EU2-77 的性能，获得了一个新的突变株 EUA20。在摇瓶培养 6 天内，其滤纸纤维素酶（FPase）活性高达 14.79 IU/mL。EUA20 的 FPase、内切葡聚糖酶、β-葡萄糖苷酶和木聚糖酶活性以及蛋白质含量分别提高了 5.73、4.35、7.34、1.80 和 2.70 倍。用预处理过的油棕空果串（OPEFB）和玉米芯粉作为底物，菌株 EUA20 的 FPase 活性分别达到了约 6.52 和 8.80 IU/ml。使用菌株 EUA20 的纤维素酶，OPEFB 和玉米芯粉的还原糖产率分别为 615.8 和 636.8 mg/g 生物质。我们的结果表明，突变株 EUA20 具有在现场生产纤维素酶以有效生物转化生物质的巨大潜力。
