Jin Yixin, Liu Yang, Antonyak Marc, Peng Xu
Department of Systems Biology and Translational Medicine, College of Medicine, Texas A&M Healthy Science Center, Temple, TX, USA.
Methods Mol Biol. 2012;843:147-54. doi: 10.1007/978-1-61779-523-7_14.
The formation of blood vessel networks is a fundamental event in vertebrate embryo development. Angiogenesis and vasculogenesis are the essential processes in vascular formation. Endothelial cells play a key role during angiogenesis and vasculogenesis, and cultured vascular endothelial cells provide an indispensable model for exploring the molecular mechanisms of angiogenesis and vasculogenesis. In this chapter, we described a protocol using PECAM-1-coated Dynabeads for the isolation of vascular endothelial cells from mouse heart and lung. This method will provide up to 10(7) endothelial cells with high purity (>85%). The isolated endothelial cells retain their in vivo characteristics, such as the expression of the cell surface markers PECAM-1 and ICAM-2.
血管网络的形成是脊椎动物胚胎发育中的一个基本事件。血管生成和血管发生是血管形成的关键过程。内皮细胞在血管生成和血管发生过程中起关键作用，培养的血管内皮细胞为探索血管生成和血管发生的分子机制提供了不可或缺的模型。在本章中，我们描述了一种使用包被有血小板内皮细胞黏附分子-1（PECAM-1）的磁珠从小鼠心脏和肺中分离血管内皮细胞的方法。该方法可提供多达10⁷个高纯度（>85%）的内皮细胞。分离出的内皮细胞保留了它们的体内特性，如细胞表面标志物PECAM-1和细胞间黏附分子-2（ICAM-2）的表达。
