Keenum D G, Semba R D, Wirasasmita S, Natadisastra G, West K P, Sommer A
Dana Center for Preventive Ophthalmology, Wilmer Ophthalmological Institute, Johns Hopkins University, Baltimore, Md.
Arch Ophthalmol. 1990 Oct;108(10):1436-41. doi: 10.1001/archopht.1990.01070120084034.
Conjunctival impression cytology was performed on 236 Indonesian preschool children, half of whom had mild xerophthalmia and half of whom were age-matched controls. We devised an applicator that applies a paper disk of fixed area to the conjunctiva with even pressure. The disk applicator was used to collect impression cytology specimens from the temporal bulbar conjunctiva of one eye while the original strip technique was used on the other eye. Mean (+/- SD) serum retinol values for children with normal and abnormal discs were 22.0 +/- 8.6 micrograms/dL and 18.0 +/- 7.2 micrograms/dL P less than .0001). Mean serum values for normal and abnormal strips were 21.7 +/- 8.6 micrograms/dL and 19.0 +/- 7.7 micrograms/dL (P less than .03). Specimens obtained with the new disk applicator corresponded more closely with serum vitamin A levels and therefore vitamin A status than those obtained with the traditional strip technique.
对236名印度尼西亚学龄前儿童进行了结膜印迹细胞学检查，其中一半患有轻度干眼症，另一半为年龄匹配的对照组。我们设计了一种涂抹器，它能以均匀的压力将固定面积的纸片应用于结膜。该圆盘涂抹器用于从一只眼的颞侧球结膜收集印迹细胞学标本，而另一只眼则采用原来的纸条技术。正常和异常圆盘儿童的平均（±标准差）血清视黄醇值分别为22.0±8.6微克/分升和18.0±7.2微克/分升（P<0.0001）。正常和异常纸条的平均血清值分别为21.7±8.6微克/分升和19.0±7.7微克/分升（P<0.03）。与传统纸条技术相比，用新的圆盘涂抹器获得的标本与血清维生素A水平以及维生素A状态的对应关系更密切。
