Department of Biochemistry, Stanford University, Stanford, CA, USA.
J Assist Reprod Genet. 2012 Feb;29(2):105-15. doi: 10.1007/s10815-011-9694-6. Epub 2012 Jan 6.
To determine the vaginal microbiome in women undergoing IVF-ET and investigate correlations with clinical outcomes.
Thirty patients had blood drawn for estradiol (E(2)) and progesterone (P(4)) at four time points during the IVF-ET cycle and at 4-6 weeks of gestation, if pregnant. Vaginal swabs were obtained in different hormonal milieu, and the vaginal microbiome determined by deep sequencing of the 16S ribosomal RNA gene.
The vaginal microbiome underwent a transition during therapy in some but not all patients. Novel bacteria were found in 33% of women tested during the treatment cycle, but not at 6-8 weeks of gestation. Diversity of species varied across different hormonal milieu, and on the day of embryo transfer correlated with outcome (live birth/no live birth). The species diversity index distinguished women who had a live birth from those who did not.
This metagenomics approach has enabled discovery of novel, previously unidentified bacterial species in the human vagina in different hormonal milieu and supports a shift in the vaginal microbiome during IVF-ET therapy using standard protocols. Furthermore, the data suggest that the vaginal microbiome on the day of embryo transfer affects pregnancy outcome.
确定接受体外受精-胚胎移植（IVF-ET）的女性的阴道微生物组，并研究其与临床结局的相关性。
30 名患者在 IVF-ET 周期的四个时间点以及怀孕 4-6 周时抽取血液进行雌二醇（E（2））和孕酮（P（4））检测。在不同的激素环境中获取阴道拭子，并通过 16S 核糖体 RNA 基因的深度测序确定阴道微生物组。
在某些但不是所有患者的治疗过程中，阴道微生物组发生了转变。在接受治疗周期检测的女性中，有 33%的女性发现了新的细菌，但在怀孕 6-8 周时没有发现。不同激素环境下的物种多样性不同，在胚胎移植日与结局（活产/无活产）相关。物种多样性指数将活产和未活产的女性区分开来。
这种宏基因组学方法能够在不同的激素环境中发现人类阴道中以前未识别的新细菌物种，并支持使用标准方案在 IVF-ET 治疗过程中阴道微生物组的转变。此外，数据表明胚胎移植日的阴道微生物组会影响妊娠结局。
