Departamento de Química Analítica y Fisicoquímica, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires-IQUIFIB, CONICET, Buenos Aires, Argentina.
Electrophoresis. 2012 Jan;33(2):334-9. doi: 10.1002/elps.201100297.
We report a new method of immobilization of gold nanoparticles (AuNPs) on a fused-silica capillary through covalent binding. The resulting modified capillary was applied to electrophoretic systems to improve the efficiency of separation and the selectivity of selected solutes. The immobilization of AuNPs on the capillary wall was performed in a very simple and fast way without requiring heating. The surface features of an AuNP-coated capillary column were determined using the scanning electron microscopy. The chromatographic properties of AuNP-coated capillaries were investigated through variation of the buffer pH and separation voltage. Effective separations of synthetic peptides mixture were obtained on the AuNP-coated capillaries. The method shows a remarkable stability since it was reused about 900 times. The capacity factor was duplicated. Therefore, this modification is stable and can be applied to different separation purposes. A complex mixture of tryptic peptide fragments of HSA was analyzed in both the bare- and the AuNP-coated capillaries. Better electrophoretic peptide profile was observed when using the AuNP-coated capillary.
我们报告了一种通过共价键将金纳米粒子（AuNPs）固定在熔融硅毛细管上的新方法。所得修饰后的毛细管应用于电泳系统，以提高分离效率和选择性。AuNPs 在毛细管壁上的固定以非常简单和快速的方式进行，而无需加热。使用扫描电子显微镜确定了涂有 AuNP 的毛细管柱的表面特征。通过改变缓冲液 pH 值和分离电压来研究 AuNP 涂覆的毛细管的色谱性能。在 AuNP 涂覆的毛细管上获得了合成肽混合物的有效分离。该方法具有显著的稳定性，因为它已重复使用约 900 次。容量因子加倍。因此，这种修饰是稳定的，可以应用于不同的分离目的。在裸毛细管和 AuNP 涂覆的毛细管中分析了 HSA 的胰蛋白酶肽片段的复杂混合物。当使用 AuNP 涂覆的毛细管时，观察到更好的电泳肽谱。
